Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Key aspects for conception and construction of co-culture models of tumor-stroma interactions
Umeå University, Faculty of Medicine, Department of Radiation Sciences. Umeå University, Faculty of Medicine, Wallenberg Centre for Molecular Medicine at Umeå University (WCMM).
Umeå University, Faculty of Medicine, Department of Radiation Sciences. Umeå University, Faculty of Medicine, Wallenberg Centre for Molecular Medicine at Umeå University (WCMM).ORCID iD: 0000-0002-5847-2778
2023 (English)In: Frontiers in Bioengineering and Biotechnology, E-ISSN 2296-4185, Vol. 11, article id 1150764Article, review/survey (Refereed) Published
Abstract [en]

The tumor microenvironment is crucial in the initiation and progression of cancers. The interplay between cancer cells and the surrounding stroma shapes the tumor biology and dictates the response to cancer therapies. Consequently, a better understanding of the interactions between cancer cells and different components of the tumor microenvironment will drive progress in developing novel, effective, treatment strategies. Co-cultures can be used to study various aspects of these interactions in detail. This includes studies of paracrine relationships between cancer cells and stromal cells such as fibroblasts, endothelial cells, and immune cells, as well as the influence of physical and mechanical interactions with the extracellular matrix of the tumor microenvironment. The development of novel co-culture models to study the tumor microenvironment has progressed rapidly over recent years. Many of these models have already been shown to be powerful tools for further understanding of the pathophysiological role of the stroma and provide mechanistic insights into tumor-stromal interactions. Here we give a structured overview of different co-culture models that have been established to study tumor-stromal interactions and what we have learnt from these models. We also introduce a set of guidelines for generating and reporting co-culture experiments to facilitate experimental robustness and reproducibility.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2023. Vol. 11, article id 1150764
Keywords [en]
cancer associated fibroblasts, cell culture models, co-culture, organoid, tumor-stroma interactions
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Cancer and Oncology
Identifiers
URN: urn:nbn:se:umu:diva-208065DOI: 10.3389/fbioe.2023.1150764ISI: 000975436300001PubMedID: 37091337Scopus ID: 2-s2.0-85153519815OAI: oai:DiVA.org:umu-208065DiVA, id: diva2:1760140
Funder
Cancerforskningsfonden i Norrland, LP20-2257Cancerforskningsfonden i Norrland, LP 21-2298Cancerforskningsfonden i Norrland, LP22-2332Swedish Research Council, 2017-01531Swedish Research Council, 2022-00855Swedish Society of Medicine, SLS-890521Region Västerbotten, RV-930167Region Västerbotten, RV-978812Knut and Alice Wallenberg FoundationMarianne and Marcus Wallenberg Foundation, MMW 2020.0189Swedish Cancer Society, 20 1339 PjFAvailable from: 2023-05-29 Created: 2023-05-29 Last updated: 2023-05-29Bibliographically approved

Open Access in DiVA

fulltext(1857 kB)61 downloads
File information
File name FULLTEXT01.pdfFile size 1857 kBChecksum SHA-512
709127508aec2f79e3eebf7ac2616dddacf50a597b049eadef14b4236000b59e44d28a9f540abe03525d8a7e86634c11d72cbb55aeec0649cc15619597908cb9
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Mason, James E.Öhlund, Daniel

Search in DiVA

By author/editor
Mason, James E.Öhlund, Daniel
By organisation
Department of Radiation SciencesWallenberg Centre for Molecular Medicine at Umeå University (WCMM)
In the same journal
Frontiers in Bioengineering and Biotechnology
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)Cancer and Oncology

Search outside of DiVA

GoogleGoogle Scholar
Total: 61 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 222 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf