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Fabricating a dielectrophoretic microfluidic device using 3D-printed moulds and silver conductive paint
Umeå University, Faculty of Science and Technology, Department of Physics. Faculty of Electrical Engineering, K. N. Toosi University of Technology, Tehran, Iran.
Umeå University, Faculty of Science and Technology, Department of Physics.ORCID iD: 0000-0002-1303-0327
Umeå University, Faculty of Science and Technology, Department of Physics.ORCID iD: 0000-0002-0496-6692
Umeå University, Faculty of Science and Technology, Department of Physics.ORCID iD: 0000-0002-0168-0197
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2023 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 13, no 1, article id 9560Article in journal (Refereed) Published
Abstract [en]

Dielectrophoresis is an electric field-based technique for moving neutral particles through a fluid. When used for particle separation, dielectrophoresis has many advantages compared to other methods, like providing label-free operation with greater control of the separation forces. In this paper, we design, build, and test a low-voltage dielectrophoretic device using a 3D printing approach. This lab-on-a-chip device fits on a microscope glass slide and incorporates microfluidic channels for particle separation. First, we use multiphysics simulations to evaluate the separation efficiency of the prospective device and guide the design process. Second, we fabricate the device in PDMS (polydimethylsiloxane) by using 3D-printed moulds that contain patterns of the channels and electrodes. The imprint of the electrodes is then filled with silver conductive paint, making a 9-pole comb electrode. Lastly, we evaluate the separation efficiency of our device by introducing a mixture of 3 μm and 10 μm polystyrene particles and tracking their progression. Our device is able to efficiently separate these particles when the electrodes are energized with ±12 V at 75 kHz. Overall, our method allows the fabrication of cheap and effective dielectrophoretic microfluidic devices using commercial off-the-shelf equipment.

Place, publisher, year, edition, pages
Springer Nature, 2023. Vol. 13, no 1, article id 9560
National Category
Fluid Mechanics and Acoustics Analytical Chemistry Other Physics Topics
Identifiers
URN: urn:nbn:se:umu:diva-209721DOI: 10.1038/s41598-023-36502-9PubMedID: 37308526Scopus ID: 2-s2.0-85161909317OAI: oai:DiVA.org:umu-209721DiVA, id: diva2:1766803
Funder
Swedish Research Council, 2019-04016Swedish Foundation for Strategic ResearchThe Kempe Foundations, JCK-1916.2Available from: 2023-06-13 Created: 2023-06-13 Last updated: 2023-06-26Bibliographically approved

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Valijam, ShayanNilsson, Daniel P. G.Malyshev, DmitryÖberg, RasmusAndersson, Magnus

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