Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Nicotinamide prevents retinal vascular dropout in a rat model of ocular hypertension and supports ocular blood supply in glaucoma patients
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Ophthalmology.
Department of Clinical Neuroscience, Division of Eye and Vision, St. Erik Eye Hospital, Karolinska Institutet, Stockholm, Sweden; Department of Ophthalmology, University of Basel, Basel, Switzerland.
Department of Clinical Neuroscience, Division of Eye and Vision, St. Erik Eye Hospital, Karolinska Institutet, Stockholm, Sweden.
Department of Clinical Neuroscience, Division of Eye and Vision, St. Erik Eye Hospital, Karolinska Institutet, Stockholm, Sweden.
Show others and affiliations
2023 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 64, article id 34Article in journal (Refereed) Published
Abstract [en]

PURPOSE. To investigate whether nicotinamide (NAM) modulates retinal vasculature in glaucoma.

METHODS. This was a prospective controlled clinical trial investigating animal and human histopathology. Participants included normotensive and ocular hypertensive rats, postmortem human ocular tissue, glaucoma patients (n = 90), and healthy controls (n = 30). The study utilized histopathology, computer-assisted retinal vasculature analysis, optical coherence tomography angiography (OCTA), and NAM treatment. The main outcome measures included retinal vascular parameters in rats as assessed by AngioTool; retinal vasculature integrity in rats and humans as assessed by histopathology, antibody-staining, and ImageJ-based measurements; and retinal perfusion density (PD) and flux index in humans as assessed by OCTA.

RESULTS. A number of vessel parameters were altered in ocular hypertension/glaucoma compared to healthy controls. NAM treatment improved the retinal vasculature in ocular hypertensive rats, with an increase in mean vessel area, percentage area covered by vessels, total vessel length, total junctions, and junction density as assessed by AngioTool (all P < 0.05); vessel wall integrity as assessed by VE-cadherin antibody staining was also improved (P < 0.01). In humans, as assessed by OCTA, increases in PD in the optic nerve head and macula complete image (0.7%, P = 0.04 and 1.0%, P = 0.002, respectively) in healthy controls, and an increase in the temporal quadrant of the macula (0.7%, P = 0.02) in glaucoma patients was seen after NAM treatment.

CONCLUSIONS. NAM can prevent retinal vascular damage in an animal model of glaucoma. After NAM treatment, glaucoma patients and healthy controls demonstrated a small increase in retinal vessel parameters as assessed by OCTA.

Place, publisher, year, edition, pages
Association for Research in Vision and Ophthalmology (ARVO) , 2023. Vol. 64, article id 34
Keywords [en]
glaucoma, histopathology, nicotinamide, optical coherence tomography angiography, retinal vasculature
National Category
Ophthalmology
Identifiers
URN: urn:nbn:se:umu:diva-218131DOI: 10.1167/iovs.64.14.34PubMedID: 38010699Scopus ID: 2-s2.0-85178055569OAI: oai:DiVA.org:umu-218131DiVA, id: diva2:1819925
Funder
Swedish Research CouncilKarolinska InstituteAvailable from: 2023-12-15 Created: 2023-12-15 Last updated: 2024-01-15Bibliographically approved

Open Access in DiVA

fulltext(5923 kB)70 downloads
File information
File name FULLTEXT01.pdfFile size 5923 kBChecksum SHA-512
43d85eca90805b21b7e81d3a618bfc63c68fb9398178ea97aed842ada88b11f3c49bb3cc4554a3e22f243fecd58849874467a1adcfa50e1a0df32cb3b6e15ac9
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Gustavsson, Simon T.Linden, ChristinaJóhannesson, Gauti

Search in DiVA

By author/editor
Gustavsson, Simon T.Linden, ChristinaJóhannesson, Gauti
By organisation
OphthalmologyWallenberg Centre for Molecular Medicine at Umeå University (WCMM)
In the same journal
Investigative Ophthalmology and Visual Science
Ophthalmology

Search outside of DiVA

GoogleGoogle Scholar
Total: 70 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 197 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf