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Escherichia coli CadB is capable of promiscuously transporting muropeptides and contributing to peptidoglycan recycling
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, GA, Athens, United States.
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).ORCID iD: 0000-0002-6848-5134
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, GA, Athens, United States.
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).ORCID iD: 0000-0001-5995-718x
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2024 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 206, no 1, article id e0036923Article in journal (Refereed) Published
Abstract [en]

The bacterial peptidoglycan (PG) cell wall is remodeled during growth and division, releasing fragments called muropeptides. Muropeptides can be internalized and reused in a process called PG recycling. Escherichia coli is highly devoted to recycling muropeptides and is known to have at least two transporters, AmpG and OppBCDF, that import them into the cytoplasm. While studying mutants lacking AmpG, we unintentionally isolated mutations that led to the altered expression of a third transporter, CadB. CadB is normally upregulated under acidic pH conditions and is an antiporter for lysine and cadaverine. Here, we explored if CadB was altering PG recycling to assist in the absence of AmpG. Surprisingly, CadB overexpression was able to restore PG recycling when both AmpG and OppBCDF were absent. CadB was found to import freed PG peptides, a subpopulation of muropeptides, through a promiscuous activity. Altogether, our data support that CadB is a third transporter capable of contributing to PG recycling.

Place, publisher, year, edition, pages
American Society for Microbiology, 2024. Vol. 206, no 1, article id e0036923
Keywords [en]
cell wall, muropeptides, peptidoglycan, peptidoglycan recycling
National Category
Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-220465DOI: 10.1128/jb.00369-23ISI: 001135641100001PubMedID: 38169298Scopus ID: 2-s2.0-85183459411OAI: oai:DiVA.org:umu-220465DiVA, id: diva2:1838721
Funder
NIH (National Institutes of Health), AI176776NIH (National Institutes of Health), AI138576NIH (National Institutes of Health), AI150098Swedish Research Council, VR2018-02823Swedish Research Council, VR2018-05882Knut and Alice Wallenberg Foundation, KAW2012.0184The Kempe FoundationsAvailable from: 2024-02-19 Created: 2024-02-19 Last updated: 2024-02-19Bibliographically approved

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Gilmore, Michael C.Cava, Felipe

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Molecular Infection Medicine Sweden (MIMS)Umeå Centre for Microbial Research (UCMR)Department of Molecular Biology (Faculty of Medicine)
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