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UV-induced spectral and morphological changes in bacterial spores for inactivation assessment
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik. Swedish Defence Research Agency (FOI), Umeå, Sweden.ORCID-id: 0000-0002-0168-0197
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik.ORCID-id: 0009-0003-0399-9569
Swedish Defence Research Agency (FOI), Umeå, Sweden.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik.ORCID-id: 0000-0002-0496-6692
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2024 (Engelska)Ingår i: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 128, nr 7, s. 1638-1646Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The ability to detect and inactivate spore-forming bacteria is of significance within, for example, industrial, healthcare, and defense sectors. Not only are stringent protocols necessary for the inactivation of spores but robust procedures are also required to detect viable spores after an inactivation assay to evaluate the procedure’s success. UV radiation is a standard procedure to inactivate spores. However, there is limited understanding regarding its impact on spores’ spectral and morphological characteristics. A further insight into these UV-induced changes can significantly improve the design of spore decontamination procedures and verification assays. This work investigates the spectral and morphological changes to Bacillus thuringiensis spores after UV exposure. Using absorbance and fluorescence spectroscopy, we observe an exponential decay in the spectral intensity of amino acids and protein structures, as well as a logistic increase in dimerized DPA with increased UV exposure on bulk spore suspensions. Additionally, using micro-Raman spectroscopy, we observe DPA release and protein degradation with increased UV exposure. More specifically, the protein backbone’s 1600–1700 cm–1 amide I band decays slower than other amino acid-based structures. Last, using electron microscopy and light scattering measurements, we observe shriveling of the spore bodies with increased UV radiation, alongside the leaking of core content and disruption of proteinaceous coat and exosporium layers. Overall, this work utilized spectroscopy and electron microscopy techniques to gain new understanding of UV-induced spore inactivation relating to spore degradation and CaDPA release. The study also identified spectroscopic indicators that can be used to determine spore viability after inactivation. These findings have practical applications in the development of new spore decontamination and inactivation validation methods.

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American Chemical Society (ACS), 2024. Vol. 128, nr 7, s. 1638-1646
Nationell ämneskategori
Mikrobiologi Analytisk kemi Annan fysik
Identifikatorer
URN: urn:nbn:se:umu:diva-221378DOI: 10.1021/acs.jpcb.3c07062PubMedID: 38326108Scopus ID: 2-s2.0-85185157140OAI: oai:DiVA.org:umu-221378DiVA, id: diva2:1839730
Forskningsfinansiär
Vetenskapsrådet, 2019-04016Kempestiftelserna, JCK-1916.2Försvarsmakten, 470-A400823
Anmärkning

Published as part of The Journal of Physical Chemistry B virtual special issue “Advances in Cellular Biophysics”.

Tillgänglig från: 2024-02-21 Skapad: 2024-02-21 Senast uppdaterad: 2024-03-06Bibliografiskt granskad

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Öberg, RasmusSil, Timir B.Johansson, Alexandra C.Malyshev, DmitryAndersson, Magnus

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Öberg, RasmusSil, Timir B.Johansson, Alexandra C.Malyshev, DmitryAndersson, MagnusAndersson, Per Ola
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Institutionen för fysikUmeå Centre for Microbial Research (UCMR)
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Journal of Physical Chemistry B
MikrobiologiAnalytisk kemiAnnan fysik

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