Nitrous oxide is a long-lived greenhouse gas. Its isotopic composition provides valuable insights into sources and sinks, and about the mechanisms of formation. A major challenge in the spectroscopic analysis of the isotopocule compositions is the availability of accurate spectroscopic parameters, particularly for the minor 15N isotopocules. In this work, we introduce high-resolution spectroscopic measurements of four isotopocules of nitrous oxide: 14N2O, 14N15NO, 15N14NO, and 15N2O in the mid-infrared range of 3300 – 3550 cm–1 using a frequency comb-based Fourier transform spectrometer. The nitrous oxide samples were obtained from a chemical synthesis involving acid-catalyzed amine-borane reduction of equimolar amounts of 15N isotopically enriched sodium nitrite and 14N sodium nitrite. The high-resolution spectra, measured in a temperature-controlled single-pass absorption cell, were used to retrieve line center frequencies and relative intensities for a total of 426 rovibrational transitions of the ν1 + ν3 band of the four isotopocules, and of the one order of magnitude weaker 2ν2 + ν3 and ν1 + ν2 + ν3 – ν2 bands in the same spectral region. We compare the determined line center frequencies and relative intensities with spectroscopic parameters available in high-resolution molecular databases. For 14N2O, 14N15NO and 15N14NO we find good agreement with the HITRAN database. The 15N2O isotopocule is missing in HITRAN, and we find that its line center frequencies in the GEISA database, the Institute of Atmospheric Optics database, as well as the Ames-1 line list deviate severely from the comb measurements.