Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Photorespiratory flux and mitochondrial contribution to energy and redox balance of barley leaf protoplasts in the light and during light-dark transitions
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).ORCID-id: 0000-0001-5900-7395
2001 (Engelska)Ingår i: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 158, nr 10, s. 1325-1332Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The contribution of mitochondrial oxidation of photorespiratory and respiratory substrates to subcellular energy and redox balance was investigated in leaf protoplasts of barley (Hordeum vulgare L.). The ATP/ADP ratios (indicating the energy balance) in chloroplasts and in tine extrachloroplast compartment were highest in the light in limiting CO2 (photorespiratory conditions), and they drastically increased after illumination if plants were pre-incubated in darkness for 24 hours. After illumination, the ATP/ADP ratio rapidly decreased in chloroplasts. The NADPH/NADP ratio (as an indicator of redox balance) in chloroplasts declined rapidly during the first seconds of darkness, then slowly increased. In limiting CO2, the ratio decreased more slowly during the first minute of darkness corresponding to post-illumination respiratory burst (PIE). During this period, the activation state of chloroplast NADP-malate dehydrogenase was higher in limiting CO2 than in saturating CO2. However, during the light-enhanced dark respiration (LEDR) period, following PIE, there were no differences in subcellular NADPH/NADP ratios in saturating and limiting CO2. A decline in malate and citrate concentrations in protoplasts and activation of mitochondrial NAD-malic enzyme were revealed during LEDR. The results presented highlight the importance of glycine oxidation in mitochondria in energization of the cytosol and chloroplasts and in maintaining redox balance in the light and during the first minute after illumination. And further, they show non-photorespiratory origin of LEDR.

Ort, förlag, år, upplaga, sidor
2001. Vol. 158, nr 10, s. 1325-1332
Nyckelord [en]
Hordeum vulgare, light-dark transition, light enhanced dark respiration, mitochondria, photorespiration, post-illumination burst
Identifikatorer
URN: urn:nbn:se:umu:diva-44655ISI: 000171799100010OAI: oai:DiVA.org:umu-44655DiVA, id: diva2:434115
Tillgänglig från: 2011-08-12 Skapad: 2011-06-09 Senast uppdaterad: 2018-06-08

Open Access i DiVA

Fulltext saknas i DiVA

Person

Gardeström, Per

Sök vidare i DiVA

Av författaren/redaktören
Gardeström, Per
Av organisationen
Institutionen för fysiologisk botanikUmeå Plant Science Centre (UPSC)
I samma tidskrift
Journal of plant physiology (Print)

Sök vidare utanför DiVA

GoogleGoogle Scholar

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 268 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf