Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Identification of regulatory regions within the intron sequence of the toy gene
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet). (Åsa Rasmuson-Lestander)
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet).
(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Abstract [en]

In Drosophila there are two paralougus Pax-6 genes, twin of eyeless (toy) and eyeless (ey) Both genes have an extensive elaborate expression pattern in the brain and ventral nerve cord but are not activated at the same time in these regions. Since both genes are expressed in several tissues during different time points in development, one might expect that more than one regulatory enhancer controls them. It is known, that eyeless is directly regulated by toy at the beginning of eye development and it has been shown that there are binding sites for regulatory proteins within the introns of the eyeless gene and in vertebrate Pax-6 genes. We investigate the transcriptional activation of toy within the intron sequences of the gene and show that there is a presumptive regulatory element in intron 2, controlling toy expression in the ventral nerve cord of embryos.

Nationell ämneskategori
Naturvetenskap
Forskningsämne
molekylärbiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-50723OAI: oai:DiVA.org:umu-50723DiVA, id: diva2:467811
Tillgänglig från: 2011-12-20 Skapad: 2011-12-19 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Ingår i avhandling
1. Regulation and function of Pax-6 during head and eye development in Drosophila melanogaster
Öppna denna publikation i ny flik eller fönster >>Regulation and function of Pax-6 during head and eye development in Drosophila melanogaster
2012 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

In Drosophila melanogaster, eyeless and twin of eyeless have important function during eye development. Mutants of both genes give a variety of eye and head phenotypes with the strongest being almost headless meaning that they lack all structures derived from the eye-antennal disc. toy the first eye specification gene expressed in the regulatory network that leads to eye formation and the gene actvates eyeless. What regulates toy is still not clear and has been the focus of this thesis. In Paper I, we analysed part of the upstream region of the toy gene to be able to drive reporter genes with the expected expression pattern in embyo and larval tissues. We found that a 1300-bp region surrounding the toy transcription start is important for correct Toy expression during embryonic and larval development. We also tested for possible redundancy between the toy and ey genes by rescue experiments on some lethal allels in both genes and found that Pax-6 proteins can substitute for each other concerning both head structures and survival. However, rescue is only partial, indicating that the proteins are not fully compatible or that the levels of expression are not sufficiently reproduced by the artificial Gal4-UAS system. Furthermore, we show that inhibition of apoptosis increased survival in strong toy mutants, but did not improve eye phenotypes. In Paper II, we searched for possibly upstream regulators of toy and found that the head gap gene empty spiracles changed the expression pattern of Toy significantly in the embryonic eye-antennal primordium. By clonal analysis and ectopic expressions, we made the conclusion that Ems acts as a repressor of toy during late embryonic development and also at later developmental stages. In Paper III, we investigate presumptive toy enhancer regions within the intron sequences of the gene. Generation and examination of transgenic lines showed that there might be an enhancer region driving toy expression in the embryonic ventral nerve cord within intron 2.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå universitet, 2012. s. 40
Nationell ämneskategori
Naturvetenskap
Forskningsämne
molekylärbiologi
Identifikatorer
urn:nbn:se:umu:diva-50742 (URN)978-91-7459-348-8 (ISBN)
Disputation
2012-01-20, KBC-huset, Stora hörsalen KB3B1, Umeå universitet, Umeå, 09:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2011-12-21 Skapad: 2011-12-20 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Person

Jacobsson, LinnRasmuson-Lestander, Åsa

Sök vidare i DiVA

Av författaren/redaktören
Jacobsson, LinnRasmuson-Lestander, Åsa
Av organisationen
Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet)
Naturvetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 279 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf