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Minimal residual disease assessment in childhood acute lymphoblastic leukaemia: a Swedish multi-centre study comparing real-time polymerase chain reaction and multicolour flow cytometry
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Medicinsk och klinisk genetik. Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Pediatrik.
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2011 (Engelska)Ingår i: British Journal of Haematology, ISSN 0007-1048, E-ISSN 1365-2141, Vol. 152, nr 6, s. 743-53Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Minimal residual disease (MRD) assessment is a powerful prognostic factor for determining the risk of relapse in childhood acute lymphoblastic leukaemia (ALL). In this Swedish multi-centre study of childhood ALL diagnosed between 2002 and 2006, the MRD levels were analysed in 726 follow-up samples in 228 children using real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged immunoglobulin/T-cell receptor genes and multicolour flow cytometry (FCM). Using an MRD threshold of 0·1%, which was the sensitivity level reached in all analyses, the concordance between RQ-PCR and FCM MRD values at day 29 was 84%. In B-cell precursor ALL, an MRD level of ≥0·1% at day 29 predicted a higher risk of bone marrow relapse (BMR) with both methods, although FCM was a better discriminator. However, considering the higher median MRD values achieved with RQ-PCR, a higher MRD cut-off (≥0·2%) improved the predictive capacity of RQ-PCR. In T-ALL, RQ-PCR was notably superior to FCM in predicting risk of BMR. That notwithstanding, MRD levels of ≥0·1%, detected by either method at day 29, could not predict isolated extramedullary relapse. In conclusion, the concordance between RQ-PCR and FCM was high and hence both methods are valuable clinical tools for identifying childhood ALL cases with increased risk of BMR.

Ort, förlag, år, upplaga, sidor
Wiley-Blackwell, 2011. Vol. 152, nr 6, s. 743-53
Nyckelord [en]
flow cytometry, RQ-PCR, rearranged IG/TCR genes, minimal residual disease, childhood acute lymphoblastic leukaemia
Nationell ämneskategori
Klinisk medicin
Identifikatorer
URN: urn:nbn:se:umu:diva-82259DOI: 10.1111/j.1365-2141.2010.08456.xPubMedID: 21250970Scopus ID: 2-s2.0-79952008832OAI: oai:DiVA.org:umu-82259DiVA, id: diva2:660264
Tillgänglig från: 2013-10-29 Skapad: 2013-10-29 Senast uppdaterad: 2023-03-24Bibliografiskt granskad

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Forestier, ErikLi, AihongGrönlund, Elisabeth

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