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Effects of articular cartilage constituents on phosphotungstic acid enhanced micro-computed tomography
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB). School of Public Health, Health Science Center of Xi'an Jiaotong University; Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, P. R. China. (Chondrogenic and Osteogenic Differentiation Group)ORCID-id: 0000-0002-6181-9904
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB). (Chondrogenic and Osteogenic Differentiation Group)ORCID-id: 0000-0002-9294-7431
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2017 (Engelska)Ingår i: PLOS ONE, E-ISSN 1932-6203, Vol. 12, nr 1, artikel-id e0171075Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Contrast-enhanced micro-computed tomography (CEμCT) with phosphotungstic acid (PTA) has shown potential for detecting collagen distribution of articular cartilage. However, the selectivity of the PTA staining to articular cartilage constituents remains to be elucidated. The aim of this study was to investigate the dependence of PTA for the collagen content in bovine articular cartilage. Adjacent bovine articular cartilage samples were treated with chondroitinase ABC and collagenase to degrade the proteoglycan and the collagen constituents in articular cartilage, respectively. Enzymatically degraded samples were compared to the untreated samples using CEμCT and reference methods, such as Fourier-transform infrared imaging. Decrease in the X-ray attenuation of PTA in articular cartilage and collagen content was observed in cartilage depth of 0-13% and deeper in tissue after collagen degradation. Increase in the X-ray attenuation of PTA was observed in the cartilage depth of 13-39% after proteoglycan degradation. The X-ray attenuation of PTA-labelled articular cartilage in CEμCT is associated mainly with collagen content but the proteoglycans have a minor effect on the X-ray attenuation of the PTA-labelled articular cartilage. In conclusion, the PTA labeling provides a feasible CEμCT method for 3D characterization of articular cartilage.

Ort, förlag, år, upplaga, sidor
2017. Vol. 12, nr 1, artikel-id e0171075
Nyckelord [en]
Collagens, cartilage, collagenases, histology, articular cartilage, safranin staining, x-ray radiography, polarized light microscopy
Nationell ämneskategori
Cell- och molekylärbiologi Ortopedi Biokemi och molekylärbiologi
Forskningsämne
biokemi; cellforskning; ortopedi
Identifikatorer
URN: urn:nbn:se:umu:diva-130896DOI: 10.1371/journal.pone.0171075ISI: 000396124700046PubMedID: 28135331Scopus ID: 2-s2.0-85011307904OAI: oai:DiVA.org:umu-130896DiVA, id: diva2:1070217
Tillgänglig från: 2017-01-31 Skapad: 2017-01-31 Senast uppdaterad: 2023-03-24Bibliografiskt granskad

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Lammi, MikkoYlärinne, JanneSaarakkala, Simo

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