Assessment of Neutrophil Chemotaxis Upon G-CSF Treatment of Healthy Stem Cell Donors and in Allogeneic Transplant RecipientsVisa övriga samt affilieringar
2018 (Engelska)Ingår i: Frontiers in Immunology, E-ISSN 1664-3224, Vol. 9, artikel-id 1968
Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
Neutrophils are crucial for the human innate immunity and constitute the majority of leukocytes in circulation. Thus, blood neutrophil counts serve as a measure for the immune system's functionality. Hematological patients often have low neutrophil counts due to disease or chemotherapy. To increase neutrophil counts and thereby preventing infections in high-risk patients, recombinant G-CSF is widely used as adjunct therapy to stimulate the maturation of neutrophils. In addition, G-CSF is utilized to recruit stem cells (SCs) into the peripheral blood of SC donors. Still, the actual functionality of neutrophils resulting from G-CSF treatment remains insufficiently understood. We tested the ex vivo functionality of neutrophils isolated from blood of G-CSF-treated healthy SC donors. We quantified chemotaxis, oxidative burst, and phagocytosis before and after treatment and detected significantly reduced chemotactic activity upon G-CSF treatment. Similarly, in vitro treatment of previously untreated neutrophils with G-CSF led to reduced chemotactic activity. In addition, we revealed that this effect persists in the allogeneic SC recipients up to 4 weeks after neutrophil engraftment. Our data indicates that neutrophil quantity, as a sole measure of immunocompetence in high-risk patients should be considered cautiously as neutrophil functionality might be affected by the primary treatment.
Ort, förlag, år, upplaga, sidor
Frontiers Media S.A., 2018. Vol. 9, artikel-id 1968
Nyckelord [en]
neutrophil, granulocyte colony stimulating factor (G-CSF), allogeneic transplant, chemotaxis, hematopoietic stern cell donor
Nationell ämneskategori
Immunologi inom det medicinska området
Identifikatorer
URN: urn:nbn:se:umu:diva-152255DOI: 10.3389/fimmu.2018.01968ISI: 000444324800001PubMedID: 30254629Scopus ID: 2-s2.0-85053168114OAI: oai:DiVA.org:umu-152255DiVA, id: diva2:1252922
Forskningsfinansiär
Västerbottens läns landsting2018-10-032018-10-032025-01-15Bibliografiskt granskad
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