Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten). Clinical Microbiology, Department of Translational Medicine, Lund University.
Visa övriga samt affilieringar
2018 (Engelska)Ingår i: Journal of Visualized Experiments, E-ISSN 1940-087X, nr 140, artikel-id e54653Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Bacteria utilize complement regulators as a means of evading the host immune response. Here, we describe protocols for evaluating the role vitronectin acquisition at the bacterial cell surface plays in resistance to the host immune system. Flow cytometry experiments identified human plasma vitronectin as a ligand for the bacterial receptor outer membrane protein H of Haemophilus influenzae type f. An enzyme-linked immunosorbent assay was employed to characterize the protein-protein interactions between purified recombinant protein H and vitronectin, and binding affinity was assessed using bio-layer interferometry. The biological importance of the binding of vitronectin to protein H at the bacterial cell surface in evasion of the host immune response was confirmed using a serum resistance assay with normal and vitronectin-depleted human serum. The importance of vitronectin in bacterial adherence was analyzed using glass slides with and without vitronectin coating, followed by Gram staining. Finally, bacterial adhesion to human alveolar epithelial cell monolayers was investigated. The protocols described here can be easily adapted to the study of any bacterial species of interest.

Ort, förlag, år, upplaga, sidor
Cambride, USA: Journal of Visualized Experiments , 2018. nr 140, artikel-id e54653
Nyckelord [en]
Immunology and Infection, Issue 140, Adhesion, Bacteria, Epithelial Cells, Infection, Protein-Protein teraction, Respiratory Tract Pathogen, Serum Resistance Assay, Vitronectin
Nationell ämneskategori
Immunologi inom det medicinska området Mikrobiologi inom det medicinska området
Identifikatorer
URN: urn:nbn:se:umu:diva-156339DOI: 10.3791/54653ISI: 000456452800109PubMedID: 30394376Scopus ID: 2-s2.0-85056265043OAI: oai:DiVA.org:umu-156339DiVA, id: diva2:1288129
Tillgänglig från: 2019-02-12 Skapad: 2019-02-12 Senast uppdaterad: 2024-01-17Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Person

Singh, Birendra

Sök vidare i DiVA

Av författaren/redaktören
Singh, Birendra
Av organisationen
Institutionen för molekylärbiologi (Medicinska fakulteten)
I samma tidskrift
Journal of Visualized Experiments
Immunologi inom det medicinska områdetMikrobiologi inom det medicinska området

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 279 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf