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Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency
School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, People's Republic of China.
Xijing Hospital, Medical University of the Air Force, Xi'an, People's Republic of China.
School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, People's Republic of China.
School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, People's Republic of China.
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2019 (Engelska)Ingår i: Glycoconjugate Journal, ISSN 0282-0080, E-ISSN 1573-4986, Vol. 36, nr 6, s. 451-459Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Kashin-Beck disease (KBD) is an endemic degenerative osteoarthropathy of uncertain etiology. Our study sought to identify a correlation between small proteoglycans decorin and biglycan expression and Kashin-Beck Disease. Immunohistochemistry was used to assess the decorin and biglycan levels in cartilage specimens from both child KBD patients, and rats fed with T-2 toxin under a selenium-deficient condition. Real-time PCR and Western blot were used to assess mRNA and protein levels of decorin and biglycan in rat cartilages, as well as in C28/I2 chondrocytes stimulated by T-2 toxin and selenium in vitro. The result showed that decorin was reduced in all zones of KBD articular cartilage, while the expression of biglycan was prominently increased in KBD cartilage samples. Increased expression of biglycan and reduced expression of decorin were observed at mRNA and protein levels in the cartilage of rats fed with T-2 toxin and selenium- deficiency plus T-2 toxin diet, when compared with the normal diet group. Moreover, In vitro stimulation of C28/I2 cells with T-2 toxin resulted in an upregulation of biglycan and downregulation of decorin, T-2 toxin induction of biglycan and decorin levels were partly rescued by selenium supplement. This study highlights the focal nature of the degenerative changes that occur in KBD cartilage and may suggest that the altered expression pattern of decorin and biglycan have an important role in the onset and pathogenesis of KBD.

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Springer, 2019. Vol. 36, nr 6, s. 451-459
Nyckelord [en]
Biglycan, Decorin, Extracellular matrix, Kashin-Beck disease, Selenium, T-2 toxin
Nationell ämneskategori
Cell- och molekylärbiologi Ortopedi Farmakologi och toxikologi
Forskningsämne
cellforskning; biokemi; ortopedi; toxikologi
Identifikatorer
URN: urn:nbn:se:umu:diva-163127DOI: 10.1007/s10719-019-09889-9ISI: 000502503800001PubMedID: 31478096Scopus ID: 2-s2.0-85072103657OAI: oai:DiVA.org:umu-163127DiVA, id: diva2:1349481
Tillgänglig från: 2019-09-09 Skapad: 2019-09-09 Senast uppdaterad: 2023-03-23Bibliografiskt granskad

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Lammi, Mikko

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Glycoconjugate Journal
Cell- och molekylärbiologiOrtopediFarmakologi och toxikologi

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