Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Subtle structural alterations in G-quadruplex DNA regulate site specificity of fluorescence light-up probes
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.ORCID-id: 0000-0002-7268-9519
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.ORCID-id: 0000-0001-7691-4392
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. Department of Biophysics, Molecular Biology & Bioinformatics, University of Calcutta, 92, APC Road, Kolkata 700009, India.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.ORCID-id: 0000-0001-6347-2169
Visa övriga samt affilieringar
2020 (Engelska)Ingår i: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 48, nr 3, s. 1108-1119Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

G-quadruplex (G4) DNA structures are linked to key biological processes and human diseases. Small molecules that target specific G4 DNA structures and signal their presence would therefore be of great value as chemical research tools with potential to further advance towards diagnostic and therapeutic developments. However, the development of these types of specific compounds remain as a great challenge. In here, we have developed a compound with ability to specifically signal a certain c-MYC G4 DNA structure through a fluorescence light-up mechanism. Despite the compound's two binding sites on the G4 DNA structure, only one of them result in the fluorescence light-up effect. This G-tetrad selectivity proved to originate from a difference in flexibility that affected the binding affinity and tilt the compound out of the planar conformation required for the fluorescence light-up mechanism. The intertwined relation between the presented factors is likely the reason for the lack of examples using rational design to develop compounds with turn-on emission that specifically target certain G4 DNA structures. However, this study shows that it is indeed possible to develop such compounds and present insights into the molecular details of specific G4 DNA recognition and signaling to advance future studies of G4 biology.

Ort, förlag, år, upplaga, sidor
Oxford University Press, 2020. Vol. 48, nr 3, s. 1108-1119
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-168878DOI: 10.1093/nar/gkz1205ISI: 000515121900015PubMedID: 31912160Scopus ID: 2-s2.0-85082145471OAI: oai:DiVA.org:umu-168878DiVA, id: diva2:1415645
Forskningsfinansiär
Vetenskapsrådet, VR-NT 2017-05235Kempestiftelserna, SMK-1632Tillgänglig från: 2020-03-19 Skapad: 2020-03-19 Senast uppdaterad: 2023-03-23Bibliografiskt granskad

Open Access i DiVA

fulltext(5417 kB)202 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 5417 kBChecksumma SHA-512
9808606a528a4696b30df0e4e7fa95bc733533254fbabb295975b98cfce21983ccb996fb78742dd05ff91bc85777c52af3b2eed794a63878244c0989a5151f5b
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMedScopus

Person

Kumar, RajendraChand, KaramBhowmik, SudiptaDas, Rabindra NathHedenström, MattiasChorell, Erik

Sök vidare i DiVA

Av författaren/redaktören
Kumar, RajendraChand, KaramBhowmik, SudiptaDas, Rabindra NathHedenström, MattiasChorell, Erik
Av organisationen
Kemiska institutionen
I samma tidskrift
Nucleic Acids Research
Biokemi och molekylärbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 202 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 398 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf