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Longitudinal dynamics of the human B cell response to the yellow fever 17D vaccine
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2020 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 117, no 12, p. 6675-6685Article in journal (Refereed) Published
Abstract [en]

A comprehensive understanding of the development and evolution of human B cell responses duced by pathogen exposure will facilitate the design of next-generation vaccines. Here, we utilized a gh-throughput single B cell cloning technology to longitudinally track the human B cell response to the llow fever virus 17D (YFV-17D) vaccine. The earlymemory B cell (MBC) response was mediated by both assical immunoglobulin M (IgM) (IgM(+)CD27(+)) and switched immunoglobulin (swIg(+)) MBC pulations; however, classical IgM MBCs waned rapidly, whereas swIg(+) and atypical IgM(+) and IgD(+) MBCs were stable over time. Affinity maturation continued for 6 to 9 mo following vaccination, providing evidence for the persistence of germinal center activity long after the period of active viral replication in peripheral blood. Finally, a substantial fraction of the neutralizing antibody response was mediated by public clones that recognize a fusion loop-proximal antigenic site within domain II of the viral envelope glycoprotein. Overall, our findings provide a framework for understanding the dynamics and complexity of human B cell responses elicited by infection and vaccination.

Place, publisher, year, edition, pages
Washington: National Academy of Science , 2020. Vol. 117, no 12, p. 6675-6685
Keywords [en]
antiviral vaccination, antibody responses, yellow fever virus, monoclonal antibody, B cell memory
National Category
Infectious Medicine Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-169775DOI: 10.1073/pnas.1921388117ISI: 000521821800054PubMedID: 32152119Scopus ID: 2-s2.0-85082339528OAI: oai:DiVA.org:umu-169775DiVA, id: diva2:1428633
Available from: 2020-05-06 Created: 2020-05-06 Last updated: 2023-03-23Bibliographically approved

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Ahlm, ClasForsell, Mattias N. E.

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Haslwanter, DeniseAhlm, ClasForsell, Mattias N. E.
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Department of Clinical Microbiology
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Proceedings of the National Academy of Sciences of the United States of America
Infectious MedicineCell and Molecular Biology

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CiteExportLink to record
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Cite
Citation style
  • apa
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More styles
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  • de-DE
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  • nn-NO
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  • Other locale
More languages
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  • asciidoc
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