Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
A Detailed Flow Cytometric Analysis of Immune Activity Profiles in Molecular Subtypes of Colorectal Cancer
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
Visa övriga samt affilieringar
2020 (Engelska)Ingår i: Cancers, ISSN 2072-6694, Vol. 12, nr 11, artikel-id 3440Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The local anti-tumour immune response has important prognostic value in colorectal cancer (CRC). In the era of immunotherapy, a better understanding of the immune response in molecular subgroups of CRC may lead to significant advances in personalised medicine. On this note, microsatellite instable (MSI) tumours have been characterised by increased immune infiltration, suggesting MSI as a marker for immune inhibitor checkpoint therapy. Here, we used flow cytometry to perform a comprehensive analysis of immune activity profiles in tumour tissues, adjacent non-malignant tissues and blood, from a cohort of 69 CRC patients. We found several signs of immune suppression in tumours compared to adjacent non-malignant tissues, including T cells more often expressing the immune checkpoint molecules programmed cell death protein (PD-1) and cytotoxic T lymphocyte-associated protein 4 (CTLA-4). We further analysed immune cell infiltration in molecular subgroups of CRC. MSI tumours were indeed found to be associated with increased immune infiltration, including increased fractions of PD-1+ T cells. No correlation was, however, found between MSI and the fraction of CTLA-4+ T cells. Interestingly, within the group of patients with microsatellite stable (MSS) tumours, some also presented with increased immune infiltration, including comparably high portions of PD-1+ T cells, but also CTLA-4+ T cells. Furthermore, no correlation was found between PD-1+ and CTLA-4+ T cells, suggesting that different tumours may, to some extent, be regulated by different immune checkpoints. We further evaluated the distribution of immune activity profiles in the consensus molecular subtypes of CRC. In conclusion, our findings suggest that different immune checkpoint inhibitors may be beneficial for selected CRC patients irrespective of MSI status. Improved predictive tools are required to identify these patients.

Ort, förlag, år, upplaga, sidor
MDPI, 2020. Vol. 12, nr 11, artikel-id 3440
Nyckelord [en]
colorectal cancer, immune activity profile, microsatellite instability, consensus molecular subtypes
Nationell ämneskategori
Cancer och onkologi
Identifikatorer
URN: urn:nbn:se:umu:diva-177781DOI: 10.3390/cancers12113440ISI: 000593599000001PubMedID: 33228141Scopus ID: 2-s2.0-85096455525OAI: oai:DiVA.org:umu-177781DiVA, id: diva2:1512381
Tillgänglig från: 2020-12-22 Skapad: 2020-12-22 Senast uppdaterad: 2023-03-23Bibliografiskt granskad

Open Access i DiVA

fulltext(1705 kB)169 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 1705 kBChecksumma SHA-512
8c185a2a9a6ba04bfd42d72b8b9bb1eebf09255afa2dfcaff41bf0d1e8061295189885d46c22cee31d8c87d9613608344f35b4e4fbaea499d27ca4c925e75675
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMedScopus

Person

Li, XingruLing, AgnesKellgren, Therese G.Lundholm, MarieLöfgren Burström, AnnaZingmark, CarlRutegård, MartinLjuslinder, IngridPalmqvist, RichardEdin, Sofia

Sök vidare i DiVA

Av författaren/redaktören
Li, XingruLing, AgnesKellgren, Therese G.Lundholm, MarieLöfgren Burström, AnnaZingmark, CarlRutegård, MartinLjuslinder, IngridPalmqvist, RichardEdin, Sofia
Av organisationen
PatologiKirurgiWallenberg centrum för molekylär medicin vid Umeå universitet (WCMM)Onkologi
I samma tidskrift
Cancers
Cancer och onkologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 169 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 368 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf