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Mode of action of Disinfection chemicals  on the bacterial spore structure and their Raman spectra
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik.
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2021 (Engelska)Ingår i: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 93, nr 6, s. 3146-3153Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Contamination of toxic spore-forming bacteria is problematic since spores can survive a plethora of disinfection chemicals and it is hard to rapidly detect if the disinfection chemical has inactivated the spores. Thus, robust decontamination strategies and reliable detection methods to identify dead from viable spores are critical. In this work, we investigate the chemical changes of Bacillus thuringiensis spores treated with sporicidal agents such as chlorine dioxide, peracetic acid, and sodium hypochlorite using laser tweezers Raman spectroscopy. We also image treated spores using SEM and TEM to verify if we can correlate structural changes in the spores with changes to their Raman spectra. We found that over 30 min, chlorine dioxide did not change the Raman spectrum or the spore structure, peracetic acid showed a time-dependent decrease in the characteristic DNA/DPA peaks and ∼20% of the spores were degraded and collapsed, and spores treated with sodium hypochlorite showed an abrupt drop in DNA and DPA peaks within 20 min and some structural damage to the exosporium. Structural changes appeared in spores after 10 min, compared to the inactivation time of the spores, which is less than a minute. We conclude that vibrational spectroscopy provides powerful means to detect changes in spores but it might be problematic to identify if spores are live or dead after a decontamination procedure.

Ort, förlag, år, upplaga, sidor
American Chemical Society (ACS), 2021. Vol. 93, nr 6, s. 3146-3153
Nationell ämneskategori
Annan fysik Biofysik
Identifikatorer
URN: urn:nbn:se:umu:diva-179119DOI: 10.1021/acs.analchem.0c04519ISI: 000620922300011Scopus ID: 2-s2.0-85100614040OAI: oai:DiVA.org:umu-179119DiVA, id: diva2:1522320
Forskningsfinansiär
Vetenskapsrådet, 2019-04016Kempestiftelserna, (JCK-1916.2Tillgänglig från: 2021-01-26 Skapad: 2021-01-26 Senast uppdaterad: 2023-09-05Bibliografiskt granskad
Ingår i avhandling
1. KNOW YOUR ENEMY: Characterizing Pathogenic Biomaterials Using Laser Tweezers
Öppna denna publikation i ny flik eller fönster >>KNOW YOUR ENEMY: Characterizing Pathogenic Biomaterials Using Laser Tweezers
2022 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Diseases caused by pathogenic agents such as bacteria and viruses result in devastating costs on personal and societal levels. However, it is not just the emergence of new diseases that is problematic. Antibiotic resistance among bacteria makes uncomplicated infections difficult and lethal. Resilient disease-causing spores spread in hospitals, the food industry, and water supplies requiring effective detection and disinfection methods. Further, we face complex neurological diseases where no effective treatment or diagnostic methods exist. Thus, we must increase our fundamental understanding of these diseases to develop effective diagnostic, detection, disinfection, and treatment methods.

Classically, the methods used for detecting and studying the underlying mechanics of pathogenic agents work on a large scale, measuring the average macroscopic behavior and properties of these pathogens. However, just as with humans, the average behavior is not always representative of individual behavior. Therefore, it is also essential to investigate the characteristics of these pathogens on a single cell or particle level. 

This thesis develops and applies optical techniques to characterize pathogenic biomaterial on a single cell or particle level. At the heart of all these studies is our Optical Tweezers (OT) instrument. OT are a tool that allows us to reach into the microscopic world and interact with it. Finally, by combining OT with other experimental techniques, we can chemically characterize biomaterials and develop assays that mimic different biological settings. Using these tools, we investigate bacterial adhesion, disinfection, and detection of pathogenic spores and proteins.

Hopefully, the insights of these studies can lessen the burden on society caused by diseases by helping others develop effective treatment, diagnostic, detection, and disinfection methods in the future. 

Ort, förlag, år, upplaga, sidor
Umeå: Umeå universitet, 2022. s. 73
Nyckelord
Optical Tweezers, Laser Tweezers, Raman Spectroscopy, Bacterial Adhesion, Biophysics, Pili, Bacterial Spores, Endospores, Oocysts, Cryptosporidium, Optics
Nationell ämneskategori
Biofysik Atom- och molekylfysik och optik
Forskningsämne
biologi; fysik
Identifikatorer
urn:nbn:se:umu:diva-192471 (URN)978-91-7855-726-4 (ISBN)978-91-7855-727-1 (ISBN)
Disputation
2022-03-11, NAT.D.410, Naturvetarhuset, Umeå, 09:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2022-02-18 Skapad: 2022-02-14 Senast uppdaterad: 2022-02-15Bibliografiskt granskad

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Malyshev, DmitryDahlberg, TobiasWiklund, KristerHenriksson, Sara

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Malyshev, DmitryDahlberg, TobiasWiklund, KristerHenriksson, SaraAndersson, Magnus
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Institutionen för fysikInstitutionen för medicinsk kemi och biofysikInstitutionen för molekylärbiologi (Medicinska fakulteten)
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Analytical Chemistry
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