Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
L-Arabinose Induces the Formation of Viable Nonproliferating Spheroplasts in Vibrio cholerae
Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-sur-Yvette, France.
Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-sur-Yvette, France.
Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS). Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).ORCID-id: 0000-0002-8349-360x
Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-sur-Yvette, France.
Visa övriga samt affilieringar
2021 (Engelska)Ingår i: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 87, nr 5, artikel-id e02305-20Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Vibrio cholerae, the agent of the deadly human disease cholera, propagates as a curved rod-shaped bacterium in warm waters. It is sensitive to cold but persists in cold waters in the form of viable but nondividing coccoidal-shaped cells. Additionally, V. cholerae is able to form nonproliferating spherical cells in response to cell wall damage. It was recently reported that L-arabinose, a component of the hemicellulose and pectin of terrestrial plants, stops the growth of V. cholerae. Here, we show that L-arabinose induces the formation of spheroplasts that lose the ability to divide and stop growing in volume over time. However, they remain viable, and upon removal of L-arabinose, they start expanding in volume, form branched structures, and give rise to cells with a normal morphology after a few divisions. We further show that WigKR, a histidine kinase/response regulator pair implicated in the induction of high-level expression of cell wall synthetic genes, prevents the lysis of the spheroplasts during growth restart. Finally, we show that the physiological perturbations result from the import and catabolic processing of L-arabinose by the V. cholerae homolog of the Escherichia coli galactose transport and catabolic system. Taken together, our results suggest that the formation of nongrowing spherical cells is a common response of vibrios exposed todetrimental conditions. They also permit us to define conditions preventing any physiological perturbation of V. cholerae when using L-arabinose to induce gene expression from the tightly regulated promoter of the Escherichia coli araBAD operon.

Ort, förlag, år, upplaga, sidor
American Society for Microbiology , 2021. Vol. 87, nr 5, artikel-id e02305-20
Nyckelord [en]
growth arrest, L-arabinose, spheroplasts, Vibrio cholerae
Nationell ämneskategori
Mikrobiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-180997DOI: 10.1128/AEM.02305-20ISI: 000618054600014Scopus ID: 2-s2.0-85101047250OAI: oai:DiVA.org:umu-180997DiVA, id: diva2:1534213
Tillgänglig från: 2021-03-05 Skapad: 2021-03-05 Senast uppdaterad: 2023-09-05Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextScopus

Person

Hernández, Sara B.Cava, Felipe

Sök vidare i DiVA

Av författaren/redaktören
Hernández, Sara B.Cava, Felipe
Av organisationen
Molekylär Infektionsmedicin, Sverige (MIMS)Institutionen för molekylärbiologi (Medicinska fakulteten)
I samma tidskrift
Applied and Environmental Microbiology
Mikrobiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 162 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf