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Single-Dilution COVID-19 Antibody Test with Qualitative and Quantitative Readouts
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States.
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY, United States.
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States.
Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, Bronx, United States; Department of Biochemistry, Albert Einstein College of Medicine, NY, Bronx, United States.
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2021 (English)In: mSphere, E-ISSN 2379-5042, Vol. 6, no 2, article id e00224-21Article in journal (Refereed) Published
Abstract [en]

The coronavirus disease 2019 (COVID-19) global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to place an immense burden on societies and health care systems. A key component of COVID-19 control efforts is serological testing to determine the community prevalence of SARS-CoV-2 exposure and quantify individual immune responses to prior SARS-CoV-2 infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents to detect SARS-CoV-2 exposure in patient blood samples with high sensitivity and specificity. We further show that this sensitive test affords the estimation of viral spike-specific IgG titers from a single sample measurement, thereby providing a simple and scalable method to measure the strength of an individual's immune response. The accuracy, adaptability, and cost-effectiveness of this test make it an excellent option for clinical deployment in the ongoing COVID-19 pandemic.IMPORTANCE Serological surveillance has become an important public health tool during the COVID-19 pandemic. Detection of protective antibodies and seroconversion after SARS-CoV-2 infection or vaccination can help guide patient care plans and public health policies. Serology tests can detect antibodies against past infections; consequently, they can help overcome the shortcomings of molecular tests, which can detect only active infections. This is important, especially when considering that many COVID-19 patients are asymptomatic. In this study, we describe an enzyme-linked immunosorbent assay (ELISA)-based qualitative and quantitative serology test developed to measure IgG and IgA antibodies against the SARS-CoV-2 spike glycoprotein. The test can be deployed using commonly available laboratory reagents and equipment and displays high specificity and sensitivity. Furthermore, we demonstrate that IgG titers in patient samples can be estimated from a single measurement, enabling the assay's use in high-throughput clinical environments.

Place, publisher, year, edition, pages
American Society for Microbiology , 2021. Vol. 6, no 2, article id e00224-21
Keywords [en]
COVID-19, IgA, IgG, laboratory diagnostic test, quantitative test, SARS-CoV-2, serology, spike protein
National Category
Infectious Medicine Immunology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-183128DOI: 10.1128/mSphere.00224-21ISI: 000663823400001Scopus ID: 2-s2.0-85105050386OAI: oai:DiVA.org:umu-183128DiVA, id: diva2:1555091
Available from: 2021-05-17 Created: 2021-05-17 Last updated: 2023-09-05Bibliographically approved

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Forsell, Mattias N. E.Ahlm, Clas

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