The first human induced pluripotent stem cell line of Kashin–Beck disease reveals involvement of heparan sulfate proteoglycan biosynthesis and PPAR pathwayVisa övriga samt affilieringar
2022 (Engelska)Ingår i: The FEBS Journal, ISSN 1742-464X, E-ISSN 1742-4658, Vol. 289, nr 1, s. 279-293Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
OBJECTIVE: Kashin-Beck disease (KBD) is an endemic osteochondropathy. Due to a lack of suitable animal or cellular disease models, the research progress on KBD has been limited. Our goal was to establish the first disease-specific human induced pluripotent stem cells (hiPSCs) cellular disease model of KBD, and to explore its etiology and pathogenesis exploiting transcriptome sequencing.
METHODS: HiPSCs were reprogrammed from dermal fibroblasts of two KBD and one healthy control donors via integration-free vectors. Subsequently, hiPSCs were differentiated into chondrocytes through three-week culture. Gene expression profiles in KBD, normal primary chondrocytes and hiPSC-derived chondrocytes were defined by RNA sequencing. A Venn diagram was constructed to show the number of shared differentially expressed genes (DEGs) between KBD and normal. Gene oncology and Kyoto Encyclopedia of Genes and Genomes annotations were performed, and six DEGs were further validated in other individuals by real-time quantitative reverse transcription PCR (RT-qPCR).
RESULTS: KBD cellular disease models were successfully established by generation of hiPSC lines. Seventeen consistent and significant DEGs present in all compared groups (KBD and normal) were identified. RT-qPCR validation gave consistent results with the sequencing data. Glycosaminoglycan biosynthesis-heparan sulfate/heparin, PPAR signaling pathway and cell adhesion molecules (CAMs) pathways were identified to be significantly altered in KBD.
CONCLUSION: Differentiated chondrocytes deriving from KBD-origin hiPSCs provide the first cellular disease model for etiological studies of KBD. This study also provides new sights into the pathogenesis and etiology of KBD and is likely to inform the development of targeted therapeutics for its treatment.
Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2022. Vol. 289, nr 1, s. 279-293
Nyckelord [en]
Chondrogenesis, Disease model, Heparan sulfate proteoglycans, Human induced pluripotent stem cells, Kashin-Beck disease
Nationell ämneskategori
Ortopedi Cell- och molekylärbiologi Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) Reumatologi och inflammation
Forskningsämne
cellforskning; ortopedi; reumatologi
Identifikatorer
URN: urn:nbn:se:umu:diva-186450DOI: 10.1111/febs.16143ISI: 000682366500001PubMedID: 34324261Scopus ID: 2-s2.0-85112624359OAI: oai:DiVA.org:umu-186450DiVA, id: diva2:1582473
2021-08-022021-08-022022-01-25Bibliografiskt granskad