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Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua
Centre for Organelle Research, Faculty of Science and Technology, University of Stavanger, Stavanger, Norway; The Norwegian Centre for Movement Disorders, Stavanger University Hospital, Stavanger, Norway.ORCID iD: 0000-0001-9347-5790
2011 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 85, no 2, p. 164-169Article in journal (Refereed) Published
Abstract [en]

The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.
Place, publisher, year, edition, pages
Elsevier, 2011. Vol. 85, no 2, p. 164-169
Keywords [en]
Microbiology (medical), Molecular Biology, Microbiology
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-188652DOI: 10.1016/j.mimet.2011.01.027ISI: 000290058000013PubMedID: 21324348Scopus ID: 2-s2.0-79953207371OAI: oai:DiVA.org:umu-188652DiVA, id: diva2:1603807
Available from: 2021-10-18 Created: 2021-10-18 Last updated: 2021-10-18Bibliographically approved

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Björkblom, Benny

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