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An atlas of the Norway spruce needle seasonal transcriptome
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).ORCID-id: 0000-0003-3858-4606
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).ORCID-id: 0000-0001-7979-8876
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC). Swedish University of Agricultural Sciences (SLU), Umeå, Sweden.ORCID-id: 0000-0002-3053-0796
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).
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2021 (Engelska)Ingår i: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 108, nr 6, s. 1815-1829Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Boreal conifers possess a tremendous ability to survive and remain evergreen during harsh winter conditions and resume growth during summer. This is enabled by coordinated regulation of major cellular functions at the level of gene expression, metabolism, and physiology. Here we present a comprehensive characterization of the annual changes in the global transcriptome of Norway spruce (Picea abies) needles as a resource to understand needle development and acclimation processes throughout the year. In young, growing needles (May 15 until June 30), cell walls, organelles, etc., were formed, and this developmental program heavily influenced the transcriptome, explained by over-represented Gene Ontology (GO) categories. Later changes in gene expression were smaller but four phases were recognized: summer (July–August), autumn (September–October), winter (November–February), and spring (March–April), where over-represented GO categories demonstrated how the needles acclimated to the various seasons. Changes in the seasonal global transcriptome profile were accompanied by differential expression of members of the major transcription factor families. We present a tentative model of how cellular activities are regulated over the year in needles of Norway spruce, which demonstrates the value of mining this dataset, accessible in ConGenIE together with advanced visualization tools.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2021. Vol. 108, nr 6, s. 1815-1829
Nyckelord [en]
conifers, Norway spruce, resource, seasonal adaptation, transcriptomics
Nationell ämneskategori
Botanik
Identifikatorer
URN: urn:nbn:se:umu:diva-188962DOI: 10.1111/tpj.15530ISI: 000709512700001PubMedID: 34624161Scopus ID: 2-s2.0-85117463119OAI: oai:DiVA.org:umu-188962DiVA, id: diva2:1606843
Forskningsfinansiär
EU, Horisont 2020, 675006VinnovaForskningsrådet FormasKnut och Alice Wallenbergs StiftelseVetenskapsrådet, 2018‐05973KempestiftelsernaTillgänglig från: 2021-10-28 Skapad: 2021-10-28 Senast uppdaterad: 2023-03-24Bibliografiskt granskad
Ingår i avhandling
1. How could Christmas trees remain evergreen?: photosynthetic acclimation of Scots pine and Norway spruce needles during winter
Öppna denna publikation i ny flik eller fönster >>How could Christmas trees remain evergreen?: photosynthetic acclimation of Scots pine and Norway spruce needles during winter
2022 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Alternativ titel[sv]
Hur kan julgranen vara grön? : fotosyntesapparatens anpassning till vinterförhållanden hos tall och gran
Abstract [en]

Plants and other green organisms harvest sunlight by green chlorophyll pigments and covertit to chemical energy (sugars) and oxygen in a process called photosynthesis providing the foundation for life on Earth. Although it is unanimously believed that oceanic phytoplanktons are the main contributors to the global photosynthesis, the contribution of coniferous boreal forests distributed across vast regions of the northern hemisphere cannot be undermined. Hence boreal forests account signifificantly for social, economical and environmental sustainability. Not only do conifers thrive in the tundra regions with extreme climate, but they also maintain their needles green over the boreal winter. A question remains; what makes them so resilient? In this respect, we aimed to understand the remarkable winter adaptation strategies in two dominant boreal coniferous species,i.e., Pinus sylvestris and Picea abies. First, we mapped the transcriptional landscape in Norway spruce (Picea abies) needles over the annual cycle. Transcriptional changes in the nascent needles reflflected a sequence of developmental processes and active vegetative growth during early summer and summer. Later after maturation, transcriptome reflflected activated defense against biotic factors and acclimationin response to abiotic environmental cues such as freezing temperatures during winter. Secondly, by monitoring the photosynthetic performance of Scot pine needles, we found that the trees face extreme stress during the early spring (Feb-Mar) when sub-zero temperatures are accompanied by high solar radiation. At this time, drastic changes occur in the thylakoid membranes of the chloroplast that allows the mixing of photosystem I and photosystem II that typically remain laterally segregated. This triggers direct energy transfer from PSII to PSI and thus protects PSII from damage. Furthermore, we found that this loss of lateral segregation may be a consequence of triple phosphorylationof Lhcb1 (Light harvesting complex1 of photosystem II). The structural changes in thylakoid membranes also lead to changes inthe thylakoid macro domain organisationand pigment protein composition. Furthermore, we discovered that while PSII is protected by direct energy transfer, the protection of PSI is provided through photoreduction of oxygen by flavodiiron proteins, which in turn allows P700 to stay in an oxidised state necessary for direct energy transfer. These coordinated cascades of changes concomitantly protect both PSI and PSII to maintain the needles green over the winter.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå University, 2022. s. 65
Nyckelord
Scots Pine, Norway spruce, Photosynthesis, Winter adaptation
Nationell ämneskategori
Biokemi och molekylärbiologi Bioinformatik och systembiologi Botanik Biofysik Evolutionsbiologi
Forskningsämne
biologisk kemi; biologi; molekylärbiologi; fysiologisk botanik
Identifikatorer
urn:nbn:se:umu:diva-194032 (URN)978-91-7855-788-2 (ISBN)978-91-7855-787-5 (ISBN)
Disputation
2022-05-20, Lilla hörsalen, KBC huset, Umeå, 09:30 (Engelska)
Opponent
Handledare
Anmärkning

On page 12, List of papers, manuscripts (in preparation): Athor names hidden due to copyright - see printed version. 

Tillgänglig från: 2022-04-29 Skapad: 2022-04-22 Senast uppdaterad: 2022-05-06Bibliografiskt granskad

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Bag, PushanLihavainen, JennaDelhomme, NicolasRiquelme, ThomasRobinson, Kathryn M.Jansson, Stefan

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Bag, PushanLihavainen, JennaDelhomme, NicolasRiquelme, ThomasRobinson, Kathryn M.Jansson, Stefan
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