Over recent decades, Indigenous knowledge (IK) systems, people, and territories have increasingly been recognized in mainstream conservation practice. However, recognition of the value of IK by governing bodies varies and is often a result of colonial and “development” history and the strength of hegemonic attitudes. Through regional case studies, this chapter explores the progress and challenges of integrating IK in conservation action which is key to narrowing the knowledge-implementation gap in this discipline. Key enabling factors allow IK integration into conservation action at national levels including: recognition of Indigenous land ownership; development and acceptance of cross-cultural or Indigenous methods; devolution of power to include Indigenous People in decision-making processes; acknowledgment of Indigenous groups and their rights; and acknowledgment of the benefits of using IK in biodiversity conservation. The regional case studies presented in this chapter suggest that the recognition of IK systems in conservation programs is greatly facilitated by adopting three pillars of Indigenous empowerment (Indigenous land ownership, acknowledgment of Indigenous peoples and their rights, and acknowledgment of the value of Indigenous knowledge systems) with concomitant benefit to narrow the knowledge-implementation gap in conservation science.