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Impaired KIN10 function restores developmental defects in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutant
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-5543-2332
Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany; Institute for Plant Sciences, Cologne Biocenter, Universität zu Köln, Köln, Germany.
Laboratory for Molecular Plant Biology, Biology Department, University of Leuven–KU Leuven, Heverlee-Leuven, Belgium; KU Leuven Plant Institute (LPI), Heverlee-Leuven, Belgium.
Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.
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2022 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 235, no 1, p. 220-233Article in journal (Refereed) Published
Abstract [en]

Sensing carbohydrate availability is essential for plants to coordinate their growth and development. In Arabidopsis thaliana, TREHALOSE 6-PHOSPHATE SYNTHASE 1 (TPS1) and its product, trehalose 6-phosphate (T6P), are important for the metabolic control of development. tps1 mutants are embryo-lethal and unable to flower when embryogenesis is rescued. T6P regulates development in part through inhibition of SUCROSE NON-FERMENTING1 RELATED KINASE1 (SnRK1).

Here, we explored the role of SnRK1 in T6P-mediated plant growth and development using a combination of a mutant suppressor screen and genetic, cellular and transcriptomic approaches.

We report nonsynonymous amino acid substitutions in the catalytic KIN10 and regulatory SNF4 subunits of SnRK1 that can restore both embryogenesis and flowering of tps1 mutant plants. The identified SNF4 point mutations disrupt the interaction with the catalytic subunit KIN10.

Contrary to the common view that the two A. thaliana SnRK1 catalytic subunits act redundantly, we found that loss-of-function mutations in KIN11 are unable to restore embryogenesis and flowering, highlighting the important role of KIN10 in T6P signalling.

Place, publisher, year, edition, pages
John Wiley & Sons, 2022. Vol. 235, no 1, p. 220-233
Keywords [en]
Arabidopsis thaliana, embryogenesis, flowering time, SnRK1 complex, T6P pathway, TPS1
National Category
Botany Genetics
Identifiers
URN: urn:nbn:se:umu:diva-193809DOI: 10.1111/nph.18104ISI: 000779406900001PubMedID: 35306666Scopus ID: 2-s2.0-85127600820OAI: oai:DiVA.org:umu-193809DiVA, id: diva2:1656482
Available from: 2022-05-06 Created: 2022-05-06 Last updated: 2022-07-12Bibliographically approved

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Zacharaki, VasilikiSkorzinski, NoemiSchmid, Markus

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