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Bacterial developmental checkpoint that directly monitors cell surface morphogenesis
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, MD, Bethesda, United States.
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, MD, Bethesda, United States.
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).ORCID iD: 0000-0002-6848-5134
Department of Biological Sciences, Virginia Tech, VA, Blacksburg, United States.
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2022 (English)In: Developmental Cell, ISSN 1534-5807, E-ISSN 1878-1551, Vol. 57, no 3, p. 344-360Article in journal (Refereed) Published
Abstract [en]

Bacillus subtilis spores are encased in two concentric shells: an outer proteinaceous “coat” and an inner peptidoglycan “cortex,” separated by a membrane. Cortex assembly depends on coat assembly initiation, but how cells achieve this coordination across the membrane is unclear. Here, we report that the protein SpoVID monitors the polymerization state of the coat basement layer via an extension to a functional intracellular LysM domain that arrests sporulation when coat assembly is initiated improperly. Whereas extracellular LysM domains bind mature peptidoglycan, SpoVID LysM binds to the membrane-bound lipid II peptidoglycan precursor. We propose that improper coat assembly exposes the SpoVID LysM domain, which then sequesters lipid II and prevents cortex assembly. SpoVID defines a widespread group of firmicute proteins with a characteristic N-terminal domain and C-terminal peptidoglycan-binding domains that might combine coat and cortex assembly roles to mediate a developmental checkpoint linking the morphogenesis of two spatially separated supramolecular structures.

Place, publisher, year, edition, pages
Elsevier, 2022. Vol. 57, no 3, p. 344-360
Keywords [en]
Clostridium, Clostridium difficile, DivIVA, FtsZ, MreB, Spindle assembly checkpoint, SPOCS domain, SpoIVA, sporulation, SpoVM
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-192246DOI: 10.1016/j.devcel.2021.12.021ISI: 000753657800007PubMedID: 35065768Scopus ID: 2-s2.0-85123866840OAI: oai:DiVA.org:umu-192246DiVA, id: diva2:1656534
Funder
NIH (National Institute of Health), R01GM138630Swedish Research CouncilKnut and Alice Wallenberg FoundationThe Kempe FoundationsAvailable from: 2022-05-06 Created: 2022-05-06 Last updated: 2023-11-10Bibliographically approved

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Gilmore, Michael C.Cava, Felipe

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Molecular Infection Medicine Sweden (MIMS)Department of Molecular Biology (Faculty of Medicine)
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