Selective isolation of hyaluronan by solid phase adsorption to silicaShow others and affiliations
2022 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 652, article id 114769Article in journal (Refereed) Published
Abstract [en]
A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.
Place, publisher, year, edition, pages
Academic Press, 2022. Vol. 652, article id 114769
Keywords [en]
Conditioned medium, Glycosaminoglycan, Hyaluronan, Isolation, Serum, Silica
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:umu:diva-196536DOI: 10.1016/j.ab.2022.114769ISI: 000815786900005PubMedID: 35660507Scopus ID: 2-s2.0-85131929748OAI: oai:DiVA.org:umu-196536DiVA, id: diva2:1669466
Funder
NIH (National Institute of Health), R43GM1314442022-06-142022-06-142023-09-05Bibliographically approved