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Selective isolation of hyaluronan by solid phase adsorption to silica
Department of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, 10010, NY, United States.
Echelon Biosciences Inc., 675 Arapeen Drive, Suite 302, Salt Lake City, 84108, UT, United States.ORCID iD: 0000-0002-0805-624X
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States.
Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, 11201, NY, United States.
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2022 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 652, article id 114769Article in journal (Refereed) Published
Abstract [en]

A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.
Place, publisher, year, edition, pages
Academic Press, 2022. Vol. 652, article id 114769
Keywords [en]
Conditioned medium, Glycosaminoglycan, Hyaluronan, Isolation, Serum, Silica
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:umu:diva-196536DOI: 10.1016/j.ab.2022.114769ISI: 000815786900005PubMedID: 35660507Scopus ID: 2-s2.0-85131929748OAI: oai:DiVA.org:umu-196536DiVA, id: diva2:1669466
Funder
NIH (National Institute of Health), R43GM131444Available from: 2022-06-14 Created: 2022-06-14 Last updated: 2023-09-05Bibliographically approved

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Hellman, Urban

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Chan, Fok VunVitelli, Teraesa M.Jung, Gyu Ik DanielAmar, AnantDeAngelis, Paul L.Hellman, UrbanCowman, Mary K.
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Analytical Biochemistry
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