Selective isolation of hyaluronan by solid phase adsorption to silicaVisa övriga samt affilieringar
2022 (Engelska)Ingår i: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 652, artikel-id 114769Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.
Ort, förlag, år, upplaga, sidor
Academic Press, 2022. Vol. 652, artikel-id 114769
Nyckelord [en]
Conditioned medium, Glycosaminoglycan, Hyaluronan, Isolation, Serum, Silica
Nationell ämneskategori
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
Identifikatorer
URN: urn:nbn:se:umu:diva-196536DOI: 10.1016/j.ab.2022.114769ISI: 000815786900005PubMedID: 35660507Scopus ID: 2-s2.0-85131929748OAI: oai:DiVA.org:umu-196536DiVA, id: diva2:1669466
Forskningsfinansiär
NIH (National Institute of Health), R43GM1314442022-06-142022-06-142023-09-05Bibliografiskt granskad