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The parasitic zoonosis, opisthorchis viverrini has been an important public health problem in many parts of the globe. In Thailand, fish-borne parasitic zoonosis is highly spread in the northern and northeastern regions, where a large impact of cholangiocarcinoma occurs, a crucial source of the liver cancer. The rare occasions reports date published about the Opisthorchiasis in the middle zone of Myanmar. In our study, a total of a few species of fish borne trematode metacercariae i.e.; three kind of small intestinal flukes, the family of Heterophyidae; Haplorchoides sp., Haplorchis pumilio, Haplorchis taichi and one species of carcinogenic liver fluke, the family of Opisthorchiidae; Opisthorchis viverrini have been detected from seven study areas from Thailand and Myanmar. The geographic information relevant with the rate of infection with vulnerable species of freshwater fishes was also posted from Thailand and Myanmar, and built a parasitic diseases combine with georeference for Geographical Information System (GIS) implementation. Furthermore, secondary descriptive analysis of the prevalence of fishborne trematodes metacercariae from countries of golden triangles (Southeast Asia) ie; Thailand, Myanmar, and Laos PDR have been created a GIS database for infection status of parasite infections. The outcome from this study may be helpful in strategies for protocol of the prevention of parasitic zoonosis in freshwater fishes reportin Thailand and Myanmar.