Multiplex functional bioluminescent reporters using gaussia luciferase fused to epitope tags in an immunobinding assay
2014 (English)In: Bioluminescent imaging: methods and protocols / [ed] Christian E. Badr, Humana Press, 2014, 1, Vol. 1098, p. 231-247Chapter in book (Refereed)
Abstract [en]
The use of Gaussia luciferase in a multiplex assay can have several advantages over the singleplex method for an experimental setup. Issues such as intersample variability, screening purposes, efficiency, and in vivo applications can be addressed using a multiplex assay. Here we describe a functional reporter multiplex method using Gaussia luciferase fused to epitope tags to identify the different reporters that are expressed. Tag specific antibodies are used to bind and separate the tagged luciferase reporters.
Place, publisher, year, edition, pages
Humana Press, 2014, 1. Vol. 1098, p. 231-247
Series
Methods in Molecular Biology (MIMB), ISSN 1064-3745, E-ISSN 1940-6029 ; 1098
Keywords [en]
Antibody binding assay, Bioluminescent imaging, Epitope tag, Gaussia luciferase, Multiplex assay, Optical imaging
National Category
Biochemistry Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-202217DOI: 10.1007/978-1-62703-718-1_18PubMedID: 24166381Scopus ID: 2-s2.0-84934442767ISBN: 978-1-62703-717-4 (print)ISBN: 978-1-4939-6067-5 (print)ISBN: 978-1-62703-718-1 (electronic)OAI: oai:DiVA.org:umu-202217DiVA, id: diva2:1724082
2023-01-052023-01-052025-02-20Bibliographically approved