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Comparison of solid and liquid fractions of pretreated Norway spruce as reductants in LPMO-supported saccharification of cellulose
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.ORCID-id: 0000-0003-2798-6298
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.ORCID-id: 0000-0003-3866-0111
2022 (Engelska)Ingår i: Frontiers in Bioengineering and Biotechnology, E-ISSN 2296-4185, Vol. 10, artikel-id 1071159Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The role of lignin in enzymatic saccharification of cellulose involving lytic polysaccharide monooxygenase (LPMO) was investigated in experiments with the solid and liquid fractions of pretreated Norway spruce from a biorefinery demonstration plant using hydrothermal pretreatment and impregnation with sulfur dioxide. Pretreated biomass before and after enzymatic saccharification was characterized using HPAEC, HPLC, Py-GC/MS, 2D-HSQC NMR, FTIR, and SEM. Chemical characterization indicated that relatively harsh pretreatment conditions resulted in that the solid phase contained no or very little hemicellulose but considerable amounts of pseudo-lignin, and that the liquid phase contained a relatively high concentration (∼5 g/L) of lignin-derived phenolics. As judged from reactions continuously supplied with either air or nitrogen gas, lignin and lignin fragments from both the solid and the liquid phases efficiently served as reductants in LPMO-supported saccharification. When air was used to promote LPMO activity, the enzymatic conversion of cellulose after 72 h was 25% higher in reactions with pretreated solids and buffer, and 14% higher in reactions with pretreatment liquid and microcrystalline cellulose. Research in this area is useful for designing efficient saccharification steps in biochemical conversion of lignocellulosic biomass.

Ort, förlag, år, upplaga, sidor
Frontiers Media S.A., 2022. Vol. 10, artikel-id 1071159
Nyckelord [en]
cellulose, enzymatic saccharification, lignin, lignocellulose bioconversion, lytic polysaccharide monooxygenase, Norway spruce, pretreatment liquid, reductant
Nationell ämneskategori
Bioenergi
Identifikatorer
URN: urn:nbn:se:umu:diva-202231DOI: 10.3389/fbioe.2022.1071159ISI: 000904323600001PubMedID: 36582841Scopus ID: 2-s2.0-85145009929OAI: oai:DiVA.org:umu-202231DiVA, id: diva2:1725175
Forskningsfinansiär
Energimyndigheten, 47516-1Bio4EnergyTillgänglig från: 2023-01-10 Skapad: 2023-01-10 Senast uppdaterad: 2023-11-06Bibliografiskt granskad

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Tang, ChaojunGandla, Madhavi LathaJönsson, Leif J.

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