Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Serological Evidence of Exposure to Onyong-Nyong and Chikungunya Viruses in Febrile Patients of Rural Taita-Taveta County and Urban Kibera Informal Settlement in Nairobi, Kenya
KAVI Institute of Clinical Research, University of Nairobi, POB 19676, Nairobi, Kenya; Department of Medical Microbiology, University of Nairobi, POB 19676, Nairobi, Kenya.
Department of Virology, University of Helsinki, Helsinki, Finland; Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland.
Department of Virology, University of Helsinki, Helsinki, Finland; HUS Diagnostic Center, HUSLAB, Virology and Immunology, Helsinki University Hospital, Helsinki, Finland.
Department of Virology, University of Helsinki, Helsinki, Finland; Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland; Department of Geosciences and Geography, University of Helsinki, Helsinki, Finland.
Show others and affiliations
2022 (English)In: Viruses, E-ISSN 1999-4915, Vol. 14, no 6, article id 1286Article in journal (Refereed) Published
Abstract [en]

Several alphaviruses, such as chikungunya (CHIKV) and Onyong-nyong (ONNV), are endemic in Kenya and often cause outbreaks in different parts of the country. We assessed the seroprevalence of alphaviruses in patients with acute febrile illness in two geographically distant areas in Kenya with no previous record of alphavirus outbreaks. Blood samples were collected from febrile patients in health facilities located in the rural Taita-Taveta County in 2016 and urban Kibera informal settlement in Nairobi in 2017 and tested for CHIKV IgG and IgM antibodies using an in-house immunofluorescence assay (IFA) and a commercial ELISA test, respectively. A subset of CHIKV IgG or IgM antibody-positive samples were further analyzed using plaque reduction neutralization tests (PRNT) for CHIKV, ONNV, and Sindbis virus. Out of 537 patients, 4 (0.7%) and 28 (5.2%) had alphavirus IgM and IgG antibodies, respectively, confirmed on PRNT. We show evidence of previous and current exposure to alphaviruses based on serological testing in areas with no recorded history of outbreaks.

Place, publisher, year, edition, pages
MDPI, 2022. Vol. 14, no 6, article id 1286
Keywords [en]
alphavirus, arbovirus, chikungunya, ELISA, febrile illness, immunofluorescence, neutralization, Onyong-nyong, seroprevalence
National Category
Infectious Medicine Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-203180DOI: 10.3390/v14061286ISI: 000818334100001PubMedID: 35746757Scopus ID: 2-s2.0-85132365824OAI: oai:DiVA.org:umu-203180DiVA, id: diva2:1727454
Funder
Academy of FinlandAvailable from: 2023-01-16 Created: 2023-01-16 Last updated: 2024-01-17Bibliographically approved

Open Access in DiVA

fulltext(1702 kB)96 downloads
File information
File name FULLTEXT01.pdfFile size 1702 kBChecksum SHA-512
7de8d46e022cf41a487e985e693ebcb7e4d5d9d30815ccc4587bcc64228a0e347be33d36e819beb0c5c6a579cf3d14f8a5c4555f92e6ddb18708b355c13207a6
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Yong-Dae, GwonEvander, Magnus

Search in DiVA

By author/editor
Yong-Dae, GwonEvander, Magnus
By organisation
Department of Clinical Microbiology
In the same journal
Viruses
Infectious MedicineMicrobiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 96 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 122 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf