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Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes
Department of Biochemistry, University of Groningen, Groningen, Netherlands.
Department of Biochemistry, University of Groningen, Groningen, Netherlands.
Department of Biochemistry, University of Groningen, Groningen, Netherlands.
Department of Biochemistry, University of Groningen, Groningen, Netherlands.
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2023 (English)In: Communications Biology, E-ISSN 2399-3642, Vol. 6, no 1, article id 51Article in journal (Refereed) Published
Abstract [en]

The human pathogen Listeria monocytogenes can cope with severe environmental challenges, for which the high molecular weight stressosome complex acts as the sensing hub in a complicated signal transduction pathway. Here, we show the dynamics and functional roles of the stressosome protein RsbR1 and its paralogue, the blue-light receptor RsbL, using photo-activated localization microscopy combined with single-particle tracking and single-molecule displacement mapping and supported by physiological studies. In live cells, RsbR1 is present in multiple states: in protomers with RsbS, large clusters of stressosome complexes, and in connection with the plasma membrane via Prli42. RsbL diffuses freely in the cytoplasm but forms clusters upon exposure to light. The clustering of RsbL is independent of the presence of Prli42. Our work provides a comprehensive view of the spatial organization and intracellular dynamics of the stressosome proteins in L. monocytogenes, which paves the way towards uncovering the stress-sensing mechanism of this signal transduction pathway.

Place, publisher, year, edition, pages
Nature Publishing Group, 2023. Vol. 6, no 1, article id 51
National Category
Biochemistry and Molecular Biology Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-204069DOI: 10.1038/s42003-023-04423-yISI: 000913781400001PubMedID: 36641529Scopus ID: 2-s2.0-85146299433OAI: oai:DiVA.org:umu-204069DiVA, id: diva2:1732200
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EU, Horizon 2020, 721456Available from: 2023-01-30 Created: 2023-01-30 Last updated: 2023-09-05Bibliographically approved

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Johansson, Jörgen

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