Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Rolling circle replication and bypass of damaged nucleotides
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
2023 (English)In: Mitochondrial DNA: methods and protocols / [ed] Thomas J. Nicholls; Jay P. Uhler; Maria Falkenberg, New York: Humana Press, 2023, Vol. 2615, p. 203-217Chapter in book (Refereed)
Abstract [en]

Faithful mitochondrial DNA (mtDNA) replication is critical for the proper function of the oxidative phosphorylation system. Problems with mtDNA maintenance, such as replication stalling upon encountering DNA damage, impair this vital function and can potentially lead to disease. An in vitro reconstituted mtDNA replication system can be used to investigate how the mtDNA replisome deals with, for example, oxidatively or UV-damaged DNA. In this chapter, we provide a detailed protocol on how to study the bypass of different types of DNA damage using a rolling circle replication assay. The assay takes advantage of purified recombinant proteins and can be adapted to the examination of various aspects of mtDNA maintenance.

Place, publisher, year, edition, pages
New York: Humana Press, 2023. Vol. 2615, p. 203-217
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 2615
Keywords [en]
DNA damage, DNA polymerase, DNA replication, Mitochondrial DNA, Rolling circle
National Category
Cell and Molecular Biology Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-205510DOI: 10.1007/978-1-0716-2922-2_15PubMedID: 36807794Scopus ID: 2-s2.0-85148677879ISBN: 9781071629215 (print)ISBN: 9781071629222 (electronic)OAI: oai:DiVA.org:umu-205510DiVA, id: diva2:1743439
Available from: 2023-03-15 Created: 2023-03-15 Last updated: 2023-07-10Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Forslund, Josefin M. E.Stojkovič, Gorazd

Search in DiVA

By author/editor
Forslund, Josefin M. E.Stojkovič, GorazdWanrooij, Sjoerd
By organisation
Department of Medical Biochemistry and Biophysics
Cell and Molecular BiologyBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
isbn
urn-nbn

Altmetric score

doi
pubmed
isbn
urn-nbn
Total: 135 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf