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A lab-on-a-chip utilizing microwaves for bacterial spore disruption and detection
Umeå University, Faculty of Science and Technology, Department of Physics. Faculty of Electrical Engineering, K. N. Toosi University of Technology, Tehran, Iran.ORCID iD: 0000-0002-4843-5164
Umeå University, Faculty of Science and Technology, Department of Physics.ORCID iD: 0000-0002-1303-0327
Umeå University, Faculty of Science and Technology, Department of Physics.ORCID iD: 0000-0002-0168-0197
Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway.ORCID iD: 0000-0001-5373-0590
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2023 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 231, article id 115284Article in journal (Refereed) Published
Abstract [en]

Bacterial spores are problematic in agriculture, the food industry, and healthcare, with the fallout costs from spore-related contamination being very high. Spores are difficult to detect since they are resistant to many of the bacterial disruption techniques used to bring out the biomarkers necessary for detection. Because of this, effective and practical spore disruption methods are desirable. In this study, we demonstrate the efficiency of a compact microfluidic lab-on-chip built around a coplanar waveguide (CPW) operating at 2.45 GHz. We show that the CPW generates an electric field hotspot of ∼10 kV/m, comparable to that of a commercial microwave oven, while using only 1.2 W of input power and thus resulting in negligible sample heating. Spores passing through the microfluidic channel are disrupted by the electric field and release calcium dipicolic acid (CaDPA), a biomarker molecule present alongside DNA in the spore core. We show that it is possible to detect this disruption in a bulk spore suspension using fluorescence spectroscopy. We then use laser tweezers Raman spectroscopy (LTRS) to show the loss of CaDPA on an individual spore level and that the loss increases with irradiation power. Only 22% of the spores contain CaDPA after exposure to 1.2 W input power, compared to 71% of the untreated control spores. Additionally, spores exposed to microwaves appear visibly disrupted when imaged using scanning electron microscopy (SEM). Overall, this study shows the advantages of using a CPW for disrupting spores for biomarker release and detection.

Place, publisher, year, edition, pages
Elsevier, 2023. Vol. 231, article id 115284
Keywords [en]
Raman spectroscopy, Fluorescence sep CaDPA, Waveguide, Biomarker, Bacillus
National Category
Other Physics Topics Other Electrical Engineering, Electronic Engineering, Information Engineering Biophysics
Identifiers
URN: urn:nbn:se:umu:diva-206257DOI: 10.1016/j.bios.2023.115284ISI: 000980707400001PubMedID: 37031508Scopus ID: 2-s2.0-85151660389OAI: oai:DiVA.org:umu-206257DiVA, id: diva2:1748097
Part of project
Biophysical and Physicochemical Fingerprinting of Single Bacterial Spores, Swedish Research Council
Funder
Swedish Research Council, 2019-04016Swedish Foundation for Strategic ResearchThe Kempe Foundations, JCK-1916.2Swedish Armed Forces, 470-A400821Available from: 2023-04-01 Created: 2023-04-01 Last updated: 2023-09-05Bibliographically approved

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Valijam, ShayanNilsson, DanielÖberg, RasmusAndersson, MagnusMalyshev, Dmitry

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Valijam, ShayanNilsson, DanielÖberg, RasmusAlbertsdóttir Jonsmoen, Unni LisePorch, AdrianAndersson, MagnusMalyshev, Dmitry
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Department of PhysicsUmeå Centre for Microbial Research (UCMR)
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Biosensors & bioelectronics
Other Physics TopicsOther Electrical Engineering, Electronic Engineering, Information EngineeringBiophysics

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