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Interaction of adipose-derived stem cells with active and dormant breast cancer cells
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi.
Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Handkirurgi. Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi.
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi.
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi.ORCID-id: 0000-0003-2596-5936
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2023 (Engelska)Ingår i: Journal of Plastic, Reconstructive & Aesthetic Surgery, ISSN 1748-6815, E-ISSN 1878-0539, Vol. 83, s. 69-76Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Although autologous fat grafting is considered a successful method for the management of contour deformities, the fat graft could potentially induce cancer reappearance by fueling dormant breast cancer cells. Our aim was to characterize the role of adipose-derived stem cells on active and dormant breast cancer cell growth.

Methods: Cobalt chloride was used to induce dormancy in MCF-7 cancer cells. Proliferation of active and dormant cancer cells was determined in the presence of adipose-derived stem cells. A proteome array was used to detect cancer-related protein expression in the cell-conditioned medium. The migration of cancer cells was measured in response to conditioned medium from the adipose-derived stem cells.

Results: The adipose-derived stem cells showed variable effects on active MCF-7 cells growth and inhibited MCF-7 proliferation after the withdrawal of cobalt chloride. Of the 84 different proteins measured in the conditioned medium, only tenascin-C was differentially expressed in the co-cultures. MCF-7 cells alone did not express tenascin-C, whereas co-cultures between MCF-7 and adipose-derived stem cells expressed more tenascin-C versus adipose-derived stem cells alone. The conditioned medium from co-cultures significantly increased the migration of the cancer cells.

Conclusions: Adipose-derived stem cells themselves neither increased the growth or migration of cancer cells, suggesting that autologous fat grafting may be oncologically safe if reconstruction is postponed until there is no evidence of active disease. However, interactions between adipose-derived stem cells and MCF-7 cancer cells could potentially lead to the production of factors, which further promote cancer cell migration.

Ort, förlag, år, upplaga, sidor
Elsevier, 2023. Vol. 83, s. 69-76
Nyckelord [en]
Adipose-derived stem cells (ASCs), Autologous fat grafting (AFG), Dormant breast cancer cells, Tenascin-C
Nationell ämneskategori
Cell- och molekylärbiologi Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
Identifikatorer
URN: urn:nbn:se:umu:diva-209546DOI: 10.1016/j.bjps.2023.05.006ISI: 001020879400001PubMedID: 37270997Scopus ID: 2-s2.0-85160658685OAI: oai:DiVA.org:umu-209546DiVA, id: diva2:1766534
Konferens
Scandinavian Association of Plastic Surgeons Congress, Reykjavik, Iceland, June 13–15, 2022
Forskningsfinansiär
Umeå universitetRegion VästerbottenTillgänglig från: 2023-06-13 Skapad: 2023-06-13 Senast uppdaterad: 2024-01-26Bibliografiskt granskad
Ingår i avhandling
1. Optimizing stem cells for reconstructive surgery
Öppna denna publikation i ny flik eller fönster >>Optimizing stem cells for reconstructive surgery
2024 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Alternativ titel[sv]
Optimering av stamceller för rekonstruktiv kirurgi
Abstract [en]

Fat grafting has become an established method in plastic surgery for treating soft tissue defects. The results for survival of the fat being transplanted is unpredictable and supplementation of the graft with the Stromal Vascular Fraction (SVF) or cultures Adipose tissue-derived stem cells (ASCs) can enhance graft viability. The ASCs are a heterogenous group of cells with various cell membrane markers, and differing growth promoting and differentiation characteristics of the stem cells derived from the fat. It is of high importance when expanding cells prior to the transplantation of the cells into patients, that the culture conditions are well defined and ideally are xenofree, avoiding use of animal-derived products. Furthermore, the procedures must be safe and not increase risk for recurrence of cancer after reconstructive surgeries. This thesis explores the phenotypic properties of a selected population of ASCs, with a view to determining their suitability for transplantation into fat grafts. ASCs were isolated from SVF of human abdominal fat and CD146+ cells were selected using immunomagnetic beads. The proliferation, angiogenic and adipogenic properties were significantly higher in the CD146+ cells. Stem cells were also isolated from lipoaspirate obtained using two different liposuction methods. Waterjet lipoaspirates yielded the greatest number of CD146+ cells with high adipogenic potential and angiogenic activity. The cells could also be successfully isolated using a closed processing system. Cells were expanded in either foetal bovine serum, platelet lysate or a chemically defined xenofree (XV) medium. Cultures in XV medium proliferated the fastest, expressed the highest number of CD146+ cells, and showed the best adipogenic and angiogenic properties. To test possible ASCs interactions with cancer cells, co-cultures with MCF-7 breast cancer cells were established. Conditioned medium from co-cultures significantly increased the migration of the cancer cells but not their proliferation, and there was increased expression of Tenascin-C in these cultures. The research in this thesis work has shown more optimal ways to isolate and expand ASCs, potentially offering new therapeutic reconstructive treatment options for a variety of medical conditions.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå University, 2024. s. 64
Serie
Umeå University medical dissertations, ISSN 0346-6612 ; 2281
Nyckelord
Adipose tissue-derived stem cells, liposuction, oncological safety, xenofree growth medium
Nationell ämneskategori
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
Forskningsämne
anatomi
Identifikatorer
urn:nbn:se:umu:diva-220039 (URN)9789180702638 (ISBN)9789180702621 (ISBN)
Disputation
2024-02-23, Sal N410, Naturvetarhuset, Umeå, 09:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2024-02-02 Skapad: 2024-01-26 Senast uppdaterad: 2024-01-29Bibliografiskt granskad

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Nyström, MariaLauvrud, Anne-TheresePérez-Díaz, SergioKingham, Paul J.Wiberg, Rebecca

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Nyström, MariaLauvrud, Anne-TheresePérez-Díaz, SergioKingham, Paul J.Wiberg, Rebecca
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Journal of Plastic, Reconstructive & Aesthetic Surgery
Cell- och molekylärbiologiMedicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

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