In this paper, we present a study for a 3D printed responsive wood interface in which we employed a scientific approach to assess the effects of various 3D printing parameters on shape-change. A full factorial design of experiments is conducted to determine the variables that maximize hygromorphic response. Analyzing the results of the experiments, we designed and fabricated origami-inspired prototypes, and tested their bimorph and gradient actuation. The contribution of this study to the growing body of literature on 3D printing responsive wood- based composites is the integration of gradient actuation and origami-inspired shape-changing strategies.