Much of the research on digitalisation in construction has been devoted to understanding the challenges and potentials of new digital technologies on an industry or firm level. Less attention has been devoted to the underlying micro-foundations needed to successfully exploit existing and explore new digital technologies, so-called digital ambidexterity. More specifically, the practices and actions of individual project practitioners and their roles in enabling digital ambidexterity needs further attention. Therefore, this article aims to increase the understanding of practitioners’ roles in enabling digital ambidexterity. Empirically, the study is based on 15 semi- structured interviews, 5 days of on-site observations, and other secondary data. The study identifies three key practitioner roles: orchestrators, ambassadors, and brokers. Also, a process model is developed that connects these practitioners and their practices and explains how digital ambidexterity is enabled. Thereby, the paper offers new insights into the micro-foundations of enabling digital ambidexterity in construction firms.