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NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Graduate School of Science and Engineering, Saitama University, Saitama, Japan.ORCID iD: 0000-0002-2285-9647
Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Japan.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
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2023 (English)In: Plant Direct, E-ISSN 2475-4455, Vol. 7, no 9, article id e529Article in journal (Refereed) Published
Abstract [en]

The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND-INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two-hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023. Vol. 7, no 9, article id e529
Keywords [en]
Arabidopsis thaliana, leaf senescence, NAC domain protein, protein–protein interaction, transcription factor
National Category
Botany Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-214775DOI: 10.1002/pld3.529PubMedID: 37731912Scopus ID: 2-s2.0-85171678868OAI: oai:DiVA.org:umu-214775DiVA, id: diva2:1801879
Available from: 2023-10-03 Created: 2023-10-03 Last updated: 2023-10-03Bibliographically approved

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Nagahage, Isura Sumeda PriyadarshanaMannapperuma, Chanaka

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