Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
TraN: A novel repressor of an Enterococcus conjugative type IV secretion system
Institute of Molecular Biosciences, University of Graz, Graz, Austria.ORCID iD: 0000-0002-1241-162X
Institute of Molecular Biosciences, University of Graz, Graz, Austria; Institute of Biophysics, Johannes Kepler University, Linz, Austria.
Institute of Molecular Biosciences, University of Graz, Graz, Austria.ORCID iD: 0000-0001-6571-2162
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Australia.
Show others and affiliations
2018 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 46, no 17, p. 9201-9219Article in journal (Refereed) Published
Abstract [en]

The dissemination of multi-resistant bacteria represents an enormous burden on modern healthcare. Plasmid-borne conjugative transfer is the most prevalent mechanism, requiring a type IV secretion system that enables bacteria to spread beneficial traits, such as resistance to last-line antibiotics, among different genera. Inc18 plasmids, like the Gram-positive broad host-range plasmid pIP501, are substantially involved in propagation of vancomycin resistance from Enterococci to methicillin-resistant strains of Staphylococcus aureus. Here, we identified the small cytosolic protein TraN as a repressor of the pIP501-encoded conjugative transfer system, since deletion of traN resulted in upregulation of transfer factors, leading to highly enhanced conjugative transfer. Furthermore, we report the complex structure of TraN with DNA and define the exact sequence of its binding motif. Targeting this protein–DNA interaction might represent a novel therapeutic approach against the spreading of antibiotic resistances.

Place, publisher, year, edition, pages
Oxford University Press, 2018. Vol. 46, no 17, p. 9201-9219
National Category
Microbiology Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-215036DOI: 10.1093/nar/gky671ISI: 000450952000049PubMedID: 30060171Scopus ID: 2-s2.0-85054344697OAI: oai:DiVA.org:umu-215036DiVA, id: diva2:1802935
Available from: 2023-10-06 Created: 2023-10-06 Last updated: 2023-10-16Bibliographically approved

Open Access in DiVA

fulltext(7731 kB)17 downloads
File information
File name FULLTEXT01.pdfFile size 7731 kBChecksum SHA-512
9a311dc7412188908009b98660671737ab641cbcaf195e561a541b48bf46dee4242619eb5a849a0eb110da19710d6d8bb4a0d47d4b06c7f53de54ecc3de4943f
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Kohler, VerenaKohler, Andreas

Search in DiVA

By author/editor
Kohler, VerenaKohler, AndreasKeller, Walter
In the same journal
Nucleic Acids Research
MicrobiologyMicrobiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 17 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 92 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf