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Secretome from myoblasts statically loaded at low intensity promotes tenocyte proliferation via the IGF-1 receptor pathway
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB).ORCID-id: 0009-0001-1276-4644
Umeå universitet, Medicinska fakulteten, Institutionen för samhällsmedicin och rehabilitering, Avdelningen för fysioterapi.ORCID-id: 0000-0002-1617-334X
School of Medicine, Southeast University, Nanjing, China.
Department of Orthopaedics, Sahlgrenska University Hospital, Gothenburg, Sweden.
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2023 (Engelska)Ingår i: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 37, nr 10, artikel-id e23203Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Exercise is widely recognized as beneficial for tendon healing. Recently, it has been described that muscle-derived molecules secreted in response to static exercise influence tendon healing. In this study, the optimal static loading intensity for tendon healing and the composition of secretome released by myoblasts in response to different intensities of static strain were investigated. In an in vitro coculture model, myoblasts were mechanically loaded using a Flexcell Tension System. Tenocytes were seeded on transwell inserts that allowed communication between the tenocytes and myoblasts without direct contact. Proliferation and migration assays, together with RNA sequencing, were used to determine potential cellular signaling pathways. The secretome from myoblasts exposed to 2% static loading increased the proliferation and migration of the cocultured tenocytes. RNA-seq analysis revealed that this loading condition upregulated the expression of numerous genes encoding secretory proteins, including insulin-like growth factor-1 (IGF-1). Confirmation of IGF-1 expression and secretion was carried out using qPCR and enzyme-linked immunosorbt assay (ELISA), revealing a statistically significant upregulation in response to 2% static loading in comparison to both control conditions and higher loading intensities of 5% and 10%. Addition of an inhibitor of the IGF-1 receptor (PQ401) to the tenocytes significantly reduced myoblast secretome-induced tenocyte proliferation. In conclusion, IGF-1 may be an important molecule in the statically loaded myoblast secretome, which is responsible for influencing tenocytes during exercise-induced healing.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2023. Vol. 37, nr 10, artikel-id e23203
Nyckelord [en]
IGF-1, mechanical loading, migration, muscle secretome, proliferation, tenocyte
Nationell ämneskategori
Cell- och molekylärbiologi Sjukgymnastik
Identifikatorer
URN: urn:nbn:se:umu:diva-214756DOI: 10.1096/fj.202301097RPubMedID: 37732638Scopus ID: 2-s2.0-85171800001OAI: oai:DiVA.org:umu-214756DiVA, id: diva2:1805689
Forskningsfinansiär
Åke Wibergs Stiftelse, M20-0236Åke Wibergs Stiftelse, M22-0008Kempestiftelserna, JCK- 2032.2Centrum för Idrottsforskning, P2022-0010Centrum för Idrottsforskning, P2023-0011Tillgänglig från: 2023-10-18 Skapad: 2023-10-18 Senast uppdaterad: 2023-10-18Bibliografiskt granskad

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Li, JunhongZhou, XinKingham, Paul J.Backman, Ludvig J.

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Li, JunhongZhou, XinKingham, Paul J.Backman, Ludvig J.
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Institutionen för integrativ medicinsk biologi (IMB)Avdelningen för fysioterapi
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Cell- och molekylärbiologiSjukgymnastik

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