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Experimental phasing opportunities for macromolecular crystallography at very long wavelengths
Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom.
Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom.
Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom.
Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom.
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2023 (Engelska)Ingår i: Communications Chemistry, E-ISSN 2399-3669, Vol. 6, nr 1, artikel-id 219Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength. Use of longer wavelengths to access the absorption edges of biologically important lighter atoms such as calcium, potassium, chlorine, sulfur and phosphorus for native-SAD phasing is attractive but technically highly challenging. The long-wavelength beamline I23 at Diamond Light Source overcomes these limitations and extends the accessible wavelength range to λ = 5.9 Å. Here we report 22 macromolecular structures solved in this extended wavelength range, using anomalous scattering from a range of elements which demonstrate the routine feasibility of lighter atom phasing. We suggest that, in light of its advantages, long-wavelength crystallography is a compelling option for experimental phasing.

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Nature Publishing Group, 2023. Vol. 6, nr 1, artikel-id 219
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Atom- och molekylfysik och optik
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URN: urn:nbn:se:umu:diva-215721DOI: 10.1038/s42004-023-01014-0PubMedID: 37828292Scopus ID: 2-s2.0-85174207867OAI: oai:DiVA.org:umu-215721DiVA, id: diva2:1809904
Tillgänglig från: 2023-11-06 Skapad: 2023-11-06 Senast uppdaterad: 2023-11-06Bibliografiskt granskad

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