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Chitin degradation by Synechococcus WH7803
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, MA, Cambridge, United States.
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, MA, Cambridge, United States.
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, MA, Cambridge, United States.
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2023 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 13, no 1, article id 19944Article in journal (Refereed) Published
Abstract [en]

Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.
Place, publisher, year, edition, pages
Springer Nature, 2023. Vol. 13, no 1, article id 19944
National Category
Botany
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URN: urn:nbn:se:umu:diva-216889DOI: 10.1038/s41598-023-47332-0ISI: 001167946400068PubMedID: 37968300Scopus ID: 2-s2.0-85176434547OAI: oai:DiVA.org:umu-216889DiVA, id: diva2:1818691
Available from: 2023-12-12 Created: 2023-12-12 Last updated: 2025-04-24Bibliographically approved

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fulltext(1124 kB)88 downloads
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Collani, Silvio

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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  • en-GB
  • en-US
  • fi-FI
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  • nn-NB
  • sv-SE
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