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Need for speed: Human fast-twitch mitochondria favor power over efficiency
Department of Women's and Children's Health, Karolinska Institute, Stockholm, Sweden; The Åstrand Laboratory, Department of Physiology, The Swedish School of Sport and Health Sciences, Nutrition and Biomechanics, Stockholm, Sweden.
Umeå universitet, Medicinska fakulteten, Institutionen för folkhälsa och klinisk medicin. The Åstrand Laboratory, Department of Physiology, The Swedish School of Sport and Health Sciences, Nutrition and Biomechanics, Stockholm, Sweden.
The Åstrand Laboratory, Department of Physiology, The Swedish School of Sport and Health Sciences, Nutrition and Biomechanics, Stockholm, Sweden.
The Åstrand Laboratory, Department of Physiology, The Swedish School of Sport and Health Sciences, Nutrition and Biomechanics, Stockholm, Sweden; Department of Clinical Sciences, Intervention and Technology, Karolinska Institute, Stockholm, Sweden.
2024 (Engelska)Ingår i: Molecular Metabolism, ISSN 2212-8778, Vol. 79, artikel-id 101854Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Objective: Human skeletal muscle consists of a mixture of slow- and fast-twitch fibers with distinct capacities for contraction mechanics, fermentation, and oxidative phosphorylation. While the divergence in mitochondrial volume favoring slow-twitch fibers is well established, data on the fiber type-specific intrinsic mitochondrial function and morphology are highly limited with existing data mainly being generated in animal models. This highlights the need for more human data on the topic.

Methods: Here, we utilized THRIFTY, a rapid fiber type identification protocol to detect, sort, and pool fast- and slow-twitch fibers within 6 h of muscle biopsy sampling. Respiration of permeabilized fast- and slow-twitch fiber pools was then analyzed with high-resolution respirometry. Using standardized western blot procedures, muscle fiber pools were subsequently analyzed for control proteins and key proteins related to respiratory capacity.

Results: Maximal complex I+II respiration was 25% higher in human slow-twitch fibers compared to fast-twitch fibers. However, per mitochondrial volume, the respiratory rate of mitochondria in fast-twitch fibers was approximately 50% higher for complex I+II, which was primarily mediated through elevated complex II respiration. Furthermore, the abundance of complex II protein and proteins regulating cristae structure were disproportionally elevated in mitochondria of the fast-twitch fibers. The difference in intrinsic respiratory rate was not reflected in fatty acid–or complex I respiration.

Conclusion: Mitochondria of human fast-twitch muscle fibers compensate for their lack of volume by substantially elevating intrinsic respiratory rate through increased reliance on complex II.

Ort, förlag, år, upplaga, sidor
Elsevier, 2024. Vol. 79, artikel-id 101854
Nyckelord [en]
Electron transport chain, Human, Mitochondria, Muscle fiber types, Respiration, Succinate
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Fysiologi
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URN: urn:nbn:se:umu:diva-218861DOI: 10.1016/j.molmet.2023.101854PubMedID: 38104652Scopus ID: 2-s2.0-85180563756OAI: oai:DiVA.org:umu-218861DiVA, id: diva2:1824351
Tillgänglig från: 2024-01-05 Skapad: 2024-01-05 Senast uppdaterad: 2024-01-05Bibliografiskt granskad

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