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Escherichia coli CadB is capable of promiscuously transporting muropeptides and contributing to peptidoglycan recycling
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, GA, Athens, United States.
Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS). Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).ORCID-id: 0000-0002-6848-5134
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, GA, Athens, United States.
Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS). Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).ORCID-id: 0000-0001-5995-718x
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2024 (Engelska)Ingår i: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 206, nr 1, artikel-id e0036923Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The bacterial peptidoglycan (PG) cell wall is remodeled during growth and division, releasing fragments called muropeptides. Muropeptides can be internalized and reused in a process called PG recycling. Escherichia coli is highly devoted to recycling muropeptides and is known to have at least two transporters, AmpG and OppBCDF, that import them into the cytoplasm. While studying mutants lacking AmpG, we unintentionally isolated mutations that led to the altered expression of a third transporter, CadB. CadB is normally upregulated under acidic pH conditions and is an antiporter for lysine and cadaverine. Here, we explored if CadB was altering PG recycling to assist in the absence of AmpG. Surprisingly, CadB overexpression was able to restore PG recycling when both AmpG and OppBCDF were absent. CadB was found to import freed PG peptides, a subpopulation of muropeptides, through a promiscuous activity. Altogether, our data support that CadB is a third transporter capable of contributing to PG recycling.

Ort, förlag, år, upplaga, sidor
American Society for Microbiology, 2024. Vol. 206, nr 1, artikel-id e0036923
Nyckelord [en]
cell wall, muropeptides, peptidoglycan, peptidoglycan recycling
Nationell ämneskategori
Mikrobiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-220465DOI: 10.1128/jb.00369-23ISI: 001135641100001PubMedID: 38169298Scopus ID: 2-s2.0-85183459411OAI: oai:DiVA.org:umu-220465DiVA, id: diva2:1838721
Forskningsfinansiär
NIH (National Institutes of Health), AI176776NIH (National Institutes of Health), AI138576NIH (National Institutes of Health), AI150098Vetenskapsrådet, VR2018-02823Vetenskapsrådet, VR2018-05882Knut och Alice Wallenbergs Stiftelse, KAW2012.0184KempestiftelsernaTillgänglig från: 2024-02-19 Skapad: 2024-02-19 Senast uppdaterad: 2024-02-19Bibliografiskt granskad

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Gilmore, Michael C.Cava, Felipe

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Gilmore, Michael C.Cava, Felipe
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Molekylär Infektionsmedicin, Sverige (MIMS)Umeå Centre for Microbial Research (UCMR)Institutionen för molekylärbiologi (Medicinska fakulteten)
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Journal of Bacteriology
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