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In vitro toxicity evaluation in A549 cells of diesel particulate matter from two different particle sampling systems and several resuspension media
Umeå universitet, Medicinska fakulteten, Institutionen för folkhälsa och klinisk medicin.
Umeå universitet, Medicinska fakulteten, Institutionen för folkhälsa och klinisk medicin. CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.ORCID-id: 0000-0002-8753-830x
CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.
CBRN Defence and Security, Swedish Defence Research Agency, Umeå, Sweden.
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2024 (Engelska)Ingår i: Journal of Applied Toxicology, ISSN 0260-437X, E-ISSN 1099-1263Artikel i tidskrift (Refereegranskat) Epub ahead of print
Abstract [en]

In urban areas, inhalation of fine particles from combustion sources such as diesel engines causes adverse health effects. For toxicity testing, a substantial amount of particulate matter (PM) is needed. Conventional sampling involves collection of PM onto substrates by filtration or inertial impaction. A major drawback to those methodologies is that the extraction process can modify the collected particles and alter their chemical composition. Moreover, prior to toxicity testing, PM samples need to be resuspended, which can alter the PM sample even further. Lastly, the choice of the resuspension medium may also impact the detected toxicological responses. In this study, we compared the toxicity profile of PM obtained from two alternative sampling systems, using in vitro toxicity assays. One system makes use of condensational growth before collection in water in an impinger – BioSampler (CG-BioSampler), and the other, a Dekati® Gravimetric Impactor (DGI), is based on inertial impaction. In addition, various methods for resuspension of DGI collected PM were compared. Tested endpoints included cytotoxicity, formation of cellular reactive oxygen species, and genotoxicity. The alternative collection and suspension methods affected different toxicological endpoints. The water/dimethyl sulfoxide mixture and cell culture medium resuspended particles, along with the CG-BioSampler sample, produced the strongest responses. The water resuspended sample from the DGI appeared least toxic. CG-BioSampler collected PM caused a clear increased response in apoptotic cell death. We conclude that the CG-BioSampler PM sampler is a promising alternative to inertial impaction sampling.

Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2024.
Nyckelord [en]
apoptosis, diesel exhaust, extraction, impinger, particulate matter, reactive oxygen species, sampling, soot, toxicity
Nationell ämneskategori
Farmakologi och toxikologi
Identifikatorer
URN: urn:nbn:se:umu:diva-224261DOI: 10.1002/jat.4616ISI: 001214370400001PubMedID: 38705171Scopus ID: 2-s2.0-85192155238OAI: oai:DiVA.org:umu-224261DiVA, id: diva2:1857677
Tillgänglig från: 2024-05-14 Skapad: 2024-05-14 Senast uppdaterad: 2024-05-14

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Uski, O.Rankin, Gregory D.Boman, ChristofferMuala, AlaBlomberg, AndersBosson, Jenny A.Sandström, Thomas

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Uski, O.Rankin, Gregory D.Boman, ChristofferMuala, AlaBlomberg, AndersBosson, Jenny A.Sandström, Thomas
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Institutionen för folkhälsa och klinisk medicinInstitutionen för tillämpad fysik och elektronik
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