Measurements of oxygen evolution in photosynthesis
2024 (English)In: Photosynthesis: methods and protocols / [ed] Sarah Covshoff, New York: Humana Press, 2024, 2, Vol. 2790, p. 133-148Chapter in book (Refereed)
Abstract [en]
This chapter compares two different techniques for monitoring photosynthetic O2 production; the wide-spread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.
Place, publisher, year, edition, pages
New York: Humana Press, 2024, 2. Vol. 2790, p. 133-148
Series
Methods in Molecular (MIMB), ISSN 1064-3745, E-ISSN 1940-6029 ; 2790
Keywords [en]
Clark-type electrode, Membrane-inlet mass spectrometry, O2 evolution, Oxygenic photosynthesis, Photosynthetic water oxidation, Photosynthetic water splitting, Photosystem II, Chlorophyll, Electrodes, Mass Spectrometry, Oxygen, Photosynthesis, Photosystem II Protein Complex, commercial phenomena, comparative study, controlled study, cost effectiveness analysis, desorption, electrochemical analysis, illumination, ion current, membrane, nonhuman, oxygen evolution, oxygen evolution reaction, pervaporation, water splitting, metabolism, procedures
National Category
Biochemistry Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-224657DOI: 10.1007/978-1-0716-3790-6_8PubMedID: 38649570Scopus ID: 2-s2.0-85191364750ISBN: 978-1-0716-3789-0 (print)ISBN: 978-1-0716-3792-0 (print)ISBN: 978-1-0716-3790-6 (electronic)OAI: oai:DiVA.org:umu-224657DiVA, id: diva2:1859586
Funder
Swedish Research Council, 2020-03809Carl Tryggers foundation 2024-05-222024-05-222025-02-20Bibliographically approved