In vivo proteolytic profiling of the type I and type II metacaspases in Chlamydomonas reinhardtii exposed to salt stressVisa övriga samt affilieringar
2024 (Engelska)Ingår i: Physiologia Plantarum, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 176, nr 3, artikel-id e14401Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
Metacaspases are cysteine proteases present in plants, fungi and protists. While the association of metacaspases with cell death is studied in a range of organisms, their native substrates are largely unknown. Here, we explored the in vivo proteolytic landscape of the two metacaspases, CrMCA-I and CrMCA-II, present in the green freshwater alga Chlamydomonas reinhardtii, using mass spectrometry-based degradomics approach, during control conditions and salt stress. Comparison between the cleavage events of CrMCA-I and CrMCA-II in metacaspase mutants revealed unique cleavage preferences and substrate specificity. Degradome analysis demonstrated the relevance of the predicted metacaspase substrates to the physiology of C. reinhardtii cells and its adaptation during salt stress. Functional enrichment analysis indicated an involvement of CrMCA-I in the catabolism of carboxylic acids, while CrMCA-II plays an important role in photosynthesis and translation. Altogether, our findings suggest distinct cellular functions of the two metacaspases in C. reinhardtii during salt stress response.
Ort, förlag, år, upplaga, sidor
John Wiley & Sons, 2024. Vol. 176, nr 3, artikel-id e14401
Nationell ämneskategori
Växtbioteknologi Botanik
Identifikatorer
URN: urn:nbn:se:umu:diva-227585DOI: 10.1111/ppl.14401ISI: 001251179800001PubMedID: 38899462Scopus ID: 2-s2.0-85196531826OAI: oai:DiVA.org:umu-227585DiVA, id: diva2:1880435
Forskningsfinansiär
Vetenskapsrådet, 2019–04472Sven och Lilly Lawskis fond för naturvetenskaplig forskningCarl Tryggers stiftelse för vetenskaplig forskning EU, Horisont 20202024-07-012024-07-012024-07-01Bibliografiskt granskad