Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Mechanical loading modulates AMPK and mTOR signaling in muscle cells
Umeå University, Faculty of Medicine, Department of Medical and Translational Biology.ORCID iD: 0000-0002-1617-334X
Umeå University, Faculty of Science and Technology, Department of Chemistry. Umeå University, Faculty of Medicine.
Umeå University, Faculty of Medicine, Department of Medical and Translational Biology. Umeå University, Faculty of Medicine, Department of Community Medicine and Rehabilitation, Section of Physiotherapy.ORCID iD: 0009-0001-1276-4644
Umeå University, Faculty of Science and Technology, Department of Chemistry. Umeå University, Faculty of Medicine.ORCID iD: 0000-0001-6870-0677
Show others and affiliations
2024 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 23, no 10, p. 4286-4295Article in journal (Refereed) Published
Abstract [en]

Skeletal muscle adaptation to exercise involves various phenotypic changes that enhance the metabolic and contractile functions. One key regulator of these adaptive responses is the activation of AMPK, which is influenced by exercise intensity. However, the mechanistic understanding of AMPK activation during exercise remains incomplete. In this study, we utilized an in vitro model to investigate the effects of mechanical loading on AMPK activation and its interaction with the mTOR signaling pathway. Proteomic analysis of muscle cells subjected to static loading (SL) revealed distinct quantitative protein alterations associated with RNA metabolism, with 10% SL inducing the most pronounced response compared to lower intensities of 5% and 2% as well as the control. Additionally, 10% SL suppressed RNA and protein synthesis while activating AMPK and inhibiting the mTOR pathway. We also found that SRSF2, necessary for pre-mRNA splicing, is regulated by AMPK and mTOR signaling, which, in turn, is regulated in an intensity-dependent manner by SL with the highest expression in 2% SL. Further examination showed that the ADP/ATP ratio was increased after 10% SL compared to the control and that SL induced changes in mitochondrial biogenesis. Furthermore, Seahorse assay results indicate that 10% SL enhances mitochondrial respiration. These findings provide novel insights into the cellular responses to mechanical loading and shed light on the intricate AMPK-mTOR regulatory network in muscle cells.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2024. Vol. 23, no 10, p. 4286-4295
Keywords [en]
ADP/ATP ratio, AMPK, exercise adaptation, mechanical loading, mitochondrial biogenesis, mTOR, protein synthesis, proteomics analysis, RNA sequencing, skeletal muscle
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-229419DOI: 10.1021/acs.jproteome.4c00242ISI: 001302852000001PubMedID: 39213513Scopus ID: 2-s2.0-85202738975OAI: oai:DiVA.org:umu-229419DiVA, id: diva2:1896120
Funder
Åke Wiberg Foundation, M20-0236Åke Wiberg Foundation, M22-0008Swedish Research Council, P2022-0010Swedish Research Council, P2023-0011Swedish Research Council, P2024-0001The Kempe Foundations, JCK-2032.2Available from: 2024-09-09 Created: 2024-09-09 Last updated: 2024-10-28Bibliographically approved

Open Access in DiVA

fulltext(5350 kB)7 downloads
File information
File name FULLTEXT02.pdfFile size 5350 kBChecksum SHA-512
cd47e2873a408bdd21970f996b3428319948744e98384a348ff022bb01e6c3d12a64bfb69977289bc08aa1c5bcc1aa334dab35e03d73e07ccf331b1e109bf0d7
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Zhou, XinZhu, ShaochunLi, JunhongMateus, AndréWilliams, ChloeGilthorpe, Jonathan D.Backman, Ludvig J.

Search in DiVA

By author/editor
Zhou, XinZhu, ShaochunLi, JunhongMateus, AndréWilliams, ChloeGilthorpe, Jonathan D.Backman, Ludvig J.
By organisation
Department of Medical and Translational BiologyDepartment of ChemistryFaculty of MedicineSection of Physiotherapy
In the same journal
Journal of Proteome Research
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 28 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 148 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf