Protocol for analyzing the function of the type VI secretion system of the oral symbiont Aggregatibacter aphrophilus in targeting pathobionts Show others and affiliations
2024 (English) In: STAR Protocols, E-ISSN 2666-1667, Vol. 5, no 4, article id 103415Article in journal (Refereed) Published
Abstract [en]
Here, we present a protocol for evaluating type VI secretion system (T6SS)dependent fitness of the oral symbiont A. aphrophilus using biofilm competition assays and metaproteomics. We describe steps for designing T6SS-specific mutants. We then detail procedures for using them in competition assays with the pathobiont A.actinomycetemcomitans and in biofilm models, analyzing metaproteomes to assess the impact of the T6SS on multiple pathobionts. The biofilm modelis designed to mimic the oral plaque ecosystem and includes seven species. For complete details on the use and execution of this protocol, please refer to Oscarsson et al.
Place, publisher, year, edition, pages Cell Press, 2024. Vol. 5, no 4, article id 103415
Keywords [en]
Bacteriology, Microbial flora, Microbial interactions
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject Microbiology
Identifiers URN: urn:nbn:se:umu:diva-231244 DOI: 10.1016/j.xpro.2024.103415 ISI: 001344680300001 PubMedID: 39460940 Scopus ID: 2-s2.0-85207348303 OAI: oai:DiVA.org:umu-231244 DiVA, id: diva2:1908738
Funder Swedish Research Council, 2017-01198, 2021-03528 Region Västerbotten, 7002667 Umeå University Karolinska Institute 2024-10-282024-10-282025-08-28 Bibliographically approved