Spruce bark beetles are chemically communicating insects whose pheromones and other volatile organic compounds (VOCs) play a crucial role in species behavior, dispersal and mass attacks on spruce trees. This literature review compiles and analyses analytical techniques for the detection and quantification of bark beetle pheromones, and their application in bioassays to assess behavioural responses. The focus is placed on VOC sampling and extraction techniques including solvent extraction, headspace methods and solid phase microextraction (SPME) as well as on the use of various adsorbents such as Porapak Q, Tenax and polydimethylsiloxane (PDMS). Furthermore, key instrumental analytical techniques within analytical chemistry are addressed, with gas chromatography combined with mass spectrometry (GC–MS) constituting the primary method for identification and quantification of pheromone components. Complementary techniques such as GC–EAD, LC–MS and FTIR are also discussed in relation to their sensitivity and selectivity. The review demonstrates that an integrated application of advanced analytical methods and biological bioassays is necessary to identify biologically active pheromone components. This methodological combination provides an important foundation for further chemicalecological research and the development of pheromone-based applications. Keywords: bark beetles, pheromone, volatiles, olfactometer, Porapak Q, bioassay and open arena bioassay